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SUMMARY:High-Throughput Single-Molecule FRET Studies with SPAD Arrays
DTSTART:20170630T110000
DTEND:20170630T120000
DTSTAMP:20260509T225701Z
UID:d4fe4a310b218710a202b6df18289df98a250d435e0ad054190bcc09
CATEGORIES:Conferences - Seminars
DESCRIPTION:Xavier Michalet\, Department of Chemistry & Biochemistry\, UCL
 A\nAbstract:\nSingle-molecule fluorescence has emerged more than two decad
 es ago as a powerful tool to investigate biological processes at the molec
 ular level. Its main advantage over traditional "bulk" techniques is the p
 ossibility to disentangle sample heterogeneity and stochasticity by either
  taking snapshots of the conformational state of individual molecules (pro
 teins\, DNA\, etc.)\, or by following the dynamics of individual molecules
  with sub-ms (and in some cases ns) resolution. However\, for most of its 
 short history\, the field has been hampered by low throughput. Scientific 
 cameras have progressed to a point where hundreds of immobilized single-mo
 lecules can be studied in parallel\, but immobilization is a big constrain
 t. Moreover\, the temporal resolution of these measurements remains limite
 d to at best a few ms\, which is too short to study fast enzyme fluctuatio
 ns.\nOver the past few years\, our group has explored several alternative 
 technologies in collaboration with detector groups\, to go beyond these li
 mitations. Here we present our recent results obtained with SPAD arrays de
 veloped using a custom technology ensuring high quantum efficiency in the 
 visible spectrum\, allowing up to a 48-fold throughput enhancement. This n
 ew technology promises to bring single-molecule spectroscopy out of the la
 b and into mainstream biotechnology applications.\nBio:\nXavier Michalet r
 eceived an Engineering Degree from Ecole Polytechnique (France)\, before w
 orking toward a Doctorate es Science in soft condensed matter under the su
 pervision of David Bensimon at the École Normale Supérieure in Paris. Af
 ter his doctorate\, he joined the group of Aaron Bensimon at the Pasteur I
 nstitute\, where he developed genetic applications of DNA molecular combin
 g. He then moved to California\, joining Shimon Weiss’ group at the Lawr
 ence Berkeley National Laboratory\, working on quantum dots applications t
 o biology. He is currently a researcher in the Weiss group at UCLA\, worki
 ng on various single-molecule biophysics topics ranging from receptor diff
 usion to protein folding and RNA transcription\, and developing new techni
 ques and detectors for single-molecule spectroscopy and microscopy.
LOCATION:CM011 https://plan.epfl.ch/theme/generalite_thm_plan_public?lang=
 fr&room=%20CM%200%2011&dim_floor=0&dim_lang=fr&baselayer_ref=grp_backgroun
 ds&tree_groups=centres_nevralgiques%2Cacces%2Cmobilite_reduite%2Cen
STATUS:CONFIRMED
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