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SUMMARY:High-Throughput Single-Molecule FRET Studies with SPAD Arrays
DTSTART:20170630T110000
DTSTAMP:20260407T045600Z
UID:9b15fd4a488bdf416aa6a3d66517def58e3c820630c92363f2fcb10b
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Xavier Michalet\, Department of Chemistry & Biochemistry\,
  UCLA\nSingle-molecule fluorescence has emerged more than two decades ago 
 as a powerful tool to investigate biological processes at the molecular le
 vel. Its main advantage over traditional "bulk" techniques is the possibil
 ity to disentangle sample heterogeneity and stochasticity by either taking
  snapshots of the conformational state of individual molecules (proteins\,
  DNA\, etc.)\, or by following the dynamics of individual molecules with s
 ub-ms (and in some cases ns) resolution. However\, for most of its short h
 istory\, the field has been hampered by low throughput. Scientific cameras
  have progressed to a point where hundreds of immobilized single-molecules
  can be studied in parallel\, but immobilization is a big constraint. More
 over\, the temporal resolution of these measurements remains limited to at
  best a few ms\, which is too short to study fast enzyme fluctuations.\n\n
 Over the past few years\, our group has explored several alternative techn
 ologies in collaboration with detector groups\, to go beyond these limitat
 ions. Here we present our recent results obtained with SPAD arrays develop
 ed using a custom technology ensuring high quantum efficiency in the visib
 le spectrum\, allowing up to a 48-fold throughput enhancement. This new te
 chnology promises to bring single-molecule spectroscopy out of the lab and
  into mainstream biotechnology applications.\n\nBio: Xavier Michalet recei
 ved an Engineering Degree from Ecole Polytechnique (France)\, before worki
 ng toward a Doctorate es Science in soft condensed matter under the superv
 ision of David Bensimon at the École Normale Supérieure in Paris. After 
 his doctorate\, he joined the group of Aaron Bensimon at the Pasteur Insti
 tute\, where he developed genetic applications of DNA molecular combing. H
 e then moved to California\, joining Shimon Weiss’ group at the Lawrence
  Berkeley National Laboratory\, working on quantum dots applications to bi
 ology.\n\nHe is currently a researcher in the Weiss group at UCLA\, workin
 g on various single-molecule biophysics topics ranging from receptor diffu
 sion to protein folding and RNA transcription\, and developing new techniq
 ues and detectors for single-molecule spectroscopy and microscopy.\n\nHost
 : Claudio Bruschini\, EPFL/STI/IMT
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STATUS:CONFIRMED
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