BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:Lectin-driven and Glycosphingolipid-dependent Construction of Endo
 cytic Pits
DTSTART:20170905T123000
DTSTAMP:20260407T095709Z
UID:46db6a84b2c1ceaf56fc006c3b9acf81586685b973839a7bce1ef546
CATEGORIES:Conferences - Seminars
DESCRIPTION:Ludger Johannes\, Ph.D.\, Institut Curie\, Paris (F)\nBIOENGIN
 EERING SEMINAR\n\nAbstract:\nSeveral endocytic processes do not require th
 e activity of clathrin\, and it has been a major question in membrane biol
 ogy to know how the plasma membrane is bent and cargo proteins are sorted 
 in these cases. Our previous studies have allowed us to propose a novel hy
 pothesis\, termed GL-Lect hypothesis: nanodomain construction by Glycosphi
 ngoLipid-binding cellular or pathological Lectins induces membrane curvatu
 re changes and drives the formation of endocytic pits for the cellular upt
 ake of glycosylated membrane proteins with critical roles in cell migratio
 n (CD44\, alpha5beta1 integrin…)\, of pathogens (polyoma viruses\, norov
 irus) or pathogenic factors (Shiga and cholera toxins). We are now analyzi
 ng how cortical actin dynamics contributes to the clustering of glycosphin
 golipid-lectin complexes on active membranes\, thereby facilitating the nu
 cleation of endocytic tubules exploiting fluctuation forces that had not b
 een linked before to endocytosis. Furthermore\, we are identifying mechani
 sms by which the GL-Lect mechanism is acutely controlled at the plasma mem
 brane. Finally\, we study how GL-Lect domain construction at the plasma me
 mbrane programs the intracellular distribution of cargo molecules\, notabl
 y via the retrograde transport route.\n\nBio:\nLudger Johannes (Ph.D.) is 
 Research Director (DR1) at INSERM. Since the beginning of his biochemistry
  undergraduate studies in 1987\, he is member of the Studienstiftung des D
 eutschen Volkes (German organization of the academically gifted)\, and sin
 ce 1993 of Boehringer Ingelheim Fonds. Between 2001 and 2013\, he directed
  the Traffic\, Signaling and Delivery Group in the Cell Biology Department
  (UMR144 CNRS) of Institut Curie. Since January 2014\, he is heading the C
 hemical Biology of Membranes and Therapeutic Delivery unit (U1143 INSERM 
 — UMR3666 CNRS). His research aims at establishing fundamental concepts 
 of endocytosis and intracellular trafficking. The Johannes group has made 
 two major contributions in this context: the discovery of a membrane traff
 icking interface between early endosomes and the Golgi apparatus\, and the
  demonstration that dynamic protein-induced glycosphingolipid reorganizati
 on acts as a driving force for membrane invagination in clathrin-independe
 nt endocytosis. These studies are well cited and have been published in se
 veral high-ranking journals\, including Cell\, Nature\, Nature Cell Biolog
 y\, Developmental Cell\, and The Journal of Cell Biology. He also aims at 
 exploiting these discoveries in fundamental membrane biology research for 
 the development of innovative cancer therapy strategies. His basic studies
  have allowed him to validate the B-subunit of Shiga toxin (STxB) as a "pi
 lot" for the delivery of therapeutic compounds to precise intracellular lo
 cations of dendritic and tumor cells (10 patent families\, 5 of which are 
 delivered in the US\, Europe and other countries). These findings are the 
 basis for a translational research program on intracellular delivery at th
 e Curie Institute\, and for the creation of biotech companies. Ludger Joha
 nnes serves on the INSERM study section on cell and developmental biology\
 , on editorial boards of several international journals (including PLoS On
 e and Traffic)\, and is EMBO member since 2012. His group is member of Lab
 Ex CelTisPhysBio\, and he currently holds an ERC senior grant (2014-2019).
LOCATION:AI 1153 https://plan.epfl.ch/?room==AI%201153
STATUS:CONFIRMED
END:VEVENT
END:VCALENDAR