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SUMMARY:Adaptation of aberration-tolerant 3D single-molecule localisation 
 for flexible analysis
DTSTART:20171207T150000
DTEND:20171207T160000
DTSTAMP:20260506T221224Z
UID:695110c1718fe3a961c45fcfc2a62ec14dc82d2c39102d02bc3a79d0
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Alistair CURD\, MPhys\, PhD Research Fellow\, School of Mo
 lecular and Cellular Biology\, Faculty of Biological Sciences\, University
  of Leeds\, UK\nA 3D single molecule localisation setup and workflow exist
 s (Palm3d\, [1]) that produces localisations with a relative insensitivity
  to the aberrations of the optical system and without requiring a model of
  the noise in the images recorded. However\, this workflow results only in
  histogram reconstructions that are inefficient for repeated reconstructio
 ns and for sub-regions of the FOV\, with limited criteria available for se
 lection of the localisations\, and with no other output of final localisat
 ion data to the user. Therefore\, we have developed a new user interface f
 or filtering\, efficient reconstruction and extraction for downstream quan
 titative analysis of the localisation data. Some examples of its use are g
 iven\, including processing of 3D PALM data that reveals the 20-nm repeati
 ng structure of  α-actinin2 in the cardiomyocyte Z-disc.
LOCATION:BSP 407 https://plan.epfl.ch/?room==BSP%20407
STATUS:CONFIRMED
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