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SUMMARY:Exploring ultrastructure in nanomaterials\, cells and biological t
 issues by polarized microscopy
DTSTART:20180409T131500
DTEND:20180409T141500
DTSTAMP:20260413T205130Z
UID:4e142020b194660ef9d4e8e83ce4e08badffeaa44145ea81d4e26dfa
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Sophie Brasselet\, Institut Fresnel\, Marseille France\n
 Fluorescence to nonlinear coherent optical microscopy can reveal important
  spatial properties in nanomaterials\, cells and biological tissues from f
 ixed situations to in vivo dynamics. While microscopy can guide interpreta
 tion through morphological observations at the sub-micrometric scale\, opt
 ical imaging cannot directly access the way molecules are organized in spe
 cific ulstrastructures\, occuring at the molecular scale. This property\, 
 which is important in many fields\, from nanomaterials engineering to biom
 echanics\, is today most often studied using electron microscopy or X ray 
 diffraction\, which are not compatible with real time imaging.\n\nWe will 
 show that reporting molecular organization in protein filaments\, aggregat
 es or lipid membranes down to the nano scale is made possible using fluore
 scence polarization resolved optical microscopy\, which takes advantage of
  the orientation-sensitive coupling between optical excitation fields and 
 molecular transition dipole moments [1]. This approach\, which can be exte
 nded to super resolution microscopy\, has revealed ultrastructural imaging
  capabilities in the cell cytoskeleton [2]. Polarized imaging has also bee
 n also applied to nonlinear optical imaging in nanostructures\, revealing 
 nanoscale plasmonic vectorial properties in metal nanostructures [3] and s
 tructrural heterogeneities in dielectric nanoparticles.\n\n[1] S. Brassele
 t \, “Polarization resolved nonlinear microscopy: application to structu
 ral molecular and biological imaging”\, Advances in Optics and Photonics
  3\, pp. 205–271 (2011)\n\n[2] C.A. Valades Cruz\, H. A. Shaban\, A. Kre
 ss\, N. Bertaux\, S. Monneret\, M. Mavrakis\, J. Savatier\, S. Brasselet\,
  Quantitative nanoscale imaging of orientational order in biological filam
 ents by polarized super-resolution microscopy\, Proc. Natl. Acad. Sci. 113
  (7) E820-E828 (2016)\n\n[3] N.K. Balla\, C. Rendon-Barraza\, L.M. Hoang\,
  P. Karpinski\, E. Bermudez\, S. Brasselet\, Polarized nonlinear nanoscopy
  of metal nanostructures\, ACS Photonics 4 (2)\, pp 292–301 (2017)\n\nBi
 o: Sophie Brasselet is an optical physicist. She obtained her Ph.D in 1997
  at University Paris-Sud\, France on nonlinear optical properties of polym
 ers and spent a two years postdoc at UCSD (1998) and Stanford University (
 1999) in the USA\, to develop single fluorescent molecules imaging in cell
 s. After six years at ENS Cachan\, France\, as an assistant professor on n
 onlinear microscopy and optical manipulation of single molecules\, she is 
 now working as a research director at Institute Fresnel\, Marseille\, Fran
 ce. For the last fifteen years\, she has developed novel nonlinear microsc
 opy and super-resolution fluorescence tools based on polarized light\, ded
 icated to nanomaterials and biomolecular structural imaging. Using polariz
 ed fluorescence imaging approaches down to the single molecule level\, she
  has pioneered optical structural investigations at the nanoscale in cells
 . She has also extended the concept to label free nonlinear imaging\, with
  the goal to develop tools for the understanding neuropathologies. These t
 echniques are now applied in a large range of fields\, including material 
 sciences.
LOCATION:MXF 1 https://plan.epfl.ch/?room==MXF%201
STATUS:CONFIRMED
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