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SUMMARY:Dynamic Signalling Cascade Activity Measurement in Live Single Cel
 ls
DTSTART:20180208T100000
DTSTAMP:20260407T201324Z
UID:6d9d90fe03b98150201c607bf5c6d3ad556b2d0e9540563c257147a7
CATEGORIES:Conferences - Seminars
DESCRIPTION:Eric Durandau\, Ph.D.\, University of Lausanne - UNIL (CH)\nBI
 OENGINEERING SEMINAR\n\nAbstract:\nAll organisms are composed of a multitu
 de of cell types\, having each a specific task to accomplish. This cell-to
 -cell heterogeneity results from a complex interpretation of intra and ext
 racellular cues by signalling cascades. Therefore\, it becomes fundamental
  to investigate the signal processing by signalling cascades to understand
  how and why individual cell choose a particular fate. Perform such study 
 requires to measure dynamically and simultaneously the activity state of m
 ultiple signalling cascade components in live single cells. To extract suc
 h information we used synthetic biology to design a synthetic protein prob
 e\, named SKARS\, which relocates from the nucleus to the cytoplasm when b
 eing specifically phosphorylated by a Mitogen-Activated Protein Kinase (MA
 PK) of interest. We applied this technology in the study of the cross-inte
 raction between the cell cycle and the mating pathway in the model organis
 m Saccharomyces cerevisiae. We use the SKARS probe as a sensor of the mati
 ng MAPKs Fus3 and Kss1 and the transcription factor Whi5 as a sensor of th
 e CDK Cdc28. By combining time-lapse fluorescent microscopy with computati
 onal analysis\, we extracted the nucleus-to-cytoplasm shuttling of the two
  probes in hundreds of cells and used this as a proxy for MAPK and CDK act
 ivity. This strategy enables to extract information about the dynamic kina
 se activity with a higher quality compared to previously used methods. We 
 observed that when cells are exposed to exogenous pheromone\, the MAPK is 
 differentially activated from one cell to another and that these different
  signal patterns are specifics of cell-cycle stages. Moreover\, single cel
 l analysis of mutant strains leads us to conclude that the model describin
 g how the cell cycle regulates the mating pathway is incomplete. Our data 
 supports the idea that the parallel measurement of multiple signalling cas
 cades is a valuable strategy enlighten very subtle pathway cross-regulatio
 n events. Moreover\, thanks to the straightforward structure of the SKARS\
 , we strongly believe that this technique can be an advantage in fields su
 ch as development or cancer research.\n\nBio:\nUniversité de Lausanne (CH
 )\nDoctorate\n2012 – 2017\n\nUniversité Grenoble Alpes (F)\nMaster 2 (M
 2)\, Immunology microbiology Infectiology\n2011 – 2012\n\nUniversité Gr
 enoble Alpes (F)\nMaster 1 (M1)\, Molecular Biology\n2010 – 2011
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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