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SUMMARY:Single Nanoparticle Analytics: from Viruses via Exosomes to Drug C
 arriers
DTSTART:20180903T121500
DTSTAMP:20260407T095818Z
UID:a891f55e85f6f3e13669a441f3ef53133b3da184e640d8fef45559d3
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Fredrik Höök\, Chalmers University of Technology\, Got
 henburg (SE)\nDISTINGUISHED LECTURE IN BIOLOGICAL ENGINEERING\n(sandwiches
  served)\n\nAbstract: \nNext generation drug-delivery vehicles aimed to ca
 rry biological drugs\, such as proteins or nucleic acids\, are often desig
 ned to mimic how natural biological nanoparticles\, such as viruses and ex
 osomes\, transfer genetic information between cells in vivo. Due to the la
 rge heterogeneity of nanoparticles irrespective of whether they are of bio
 logical or artificial origin\, it is crucially important to advance analyt
 ical instrumentation to complement ensemble averaging methods[1] with sing
 le nanoparticle analytical approaches. A large set of tools with single-na
 noparticle sensitivity is now available\, to which we recently contributed
  a concept that enables simultaneous fluorescent and scattering-based labe
 l-free imaging of surface-bound biological nanoparticles [2]. Examples wil
 l be shown that illustrate the use of this scattering microscopy concept t
 o i) investigate supported lipid bilayer formation\, ii) label-free measur
 ements of protein binding to individual liposomes\, iii) characterize DLVO
 -controlled non-specific interactions at cell-membrane mimics\,[3] and det
 ergent free enrichment of pre-defined membrane proteins in crude cell memb
 ranes.[4] By using a two dimensional fluid supported lipid bilayer\, to wh
 ich biological nanoparticles are directly anchored and imaged\, we have al
 so  developed a new means to simultaneously determine both nanoparticle s
 ize and fluorescence / scattering intensity\,[5] which may potentially off
 er flow-cytometry-like sorting based on distinct features of individual na
 noparticles. This 2D flow nanometry concept will be discussed in the conte
 xt of improved characterization of individual biological nanoparticles of 
 diagnostic and therapeutic significance.\n\nReferences\n[1] Rupert\, D. et
  al. Analytical Chemistry 88\, 9980 (2016)\n[2] Agnarsson\, B. A. et al. A
 CS Nano 9\, 11849 (2015).\n[3] Lundgren\, A. et al. ACS Nano 10\, 9974 (20
 16).\n[4] Lundgren\, A. et al. Nano Letters 18\, 381 (2018)\n[5] Block\, S
 . et al. Nature Communication 7\, 12956 (2016)\n\nBio:\nFredrik Höök rec
 eived a PhD in physics from Chalmers\, Sweden\, in 1997 and his research h
 as focused on the development of surface-based bioanalytical tools\, such 
 as QCM-D\, localized SPR and more recently TIRF microscopy\, and their com
 bination with microfluidics for fundamental studies of cell-membrane mimic
 s but also for use in diagnostic and drug-screening applications. Efforts 
 are presently focused on (i) new methods with single molecule sensitivity 
 for studies of membrane proteins and virus infection\, (ii) microfluidic p
 latforms for cell-membrane chromatography and (iii) sophisticated surface 
 modifications and nanofabrication schemes to facilitate improved analysis 
 of supported cell-membrane mimics\, membrane proteins and virus binding an
 d infection. He is co-founder of Q-Sense\, www.q-sense.com\, who is a lead
  developer of QCM-D instruments. He has been awarded an Individual Grant f
 or the Advancement of Research Leaders by the Swedish Foundation for Strat
 egic Research in 2006 and the Göran Gustafsson prize in Physics by the Ro
 yal Swedish Society of Academy in 2012.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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