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SUMMARY:Chiral Sum Frequency Generation Spectroscopy for Protein Character
 ization at Interfaces
DTSTART:20181022T121500
DTSTAMP:20260408T071352Z
UID:8f3cfd682f89f4be6f31f6f720f99a21e98a8266f1bb1b77be9e411f
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Elsa C. Yan\, Yale University\, New Haven\, CT (USA)\nDI
 STINGUISHED LECTURE IN BIOLOGICAL ENGINEERING\n(sandwiches served)\n\nAbst
 ract: \nCharacterization of protein secondary structures at interfaces in 
 situ and in real time is important to understand biological processes asso
 ciated with cell membranes and solve problems in various fields of biomedi
 cal sciences. However\, such characterization is challenging because it re
 quires methods that are both selective to interfaces and protein secondary
  structures. We demonstrate that chiral sum frequency generation spectrosc
 opy (SFG) can provide peptide amide I and N-H stretch vibrational signals 
 that are free of water background and characteristic to parallel beta-shee
 t\, anti-parallel beta-sheet\, alpha-helix\, 3-10 helix and disordered str
 uctures\, enabling chiral SFG to distinguish protein secondary structures 
 at interfaces\, similar to circular dichroism spectroscopy for protein cha
 racterization in solution. Using chiral SFG\, we monitored misfolding of 
 an amyloid protein\, characterized protein orientation at interfaces\, dev
 eloped new methods for probing proton exchange in proteins\, and studied t
 wo-dimensional crowding effects on protein folding at interfaces. These st
 udies demonstrate chiral SFG as a new spectroscopic tool for characterizin
 g protein structures and functions at interfaces\, which can be used to ad
 dress a wide range of fundamental and engineering problems.\n \nBio:\nEls
 a Yan was born and grew up in Hong Kong. She graduated from the Chinese Un
 iversity of Hong Kong in 1995. Working with Kenneth Eisenthal on nonlinear
  optics and surface sciences\, she obtained her Ph.D. at Columbia Universi
 ty in 2000. From 2000-2004\, she was a postdoctoral fellow in Richard Math
 ies's lab at UC Berkeley and a visiting fellow in Thomas Sakmar's lab at t
 he Rockefeller University. She combined Raman spectroscopy with techniques
  in molecular biology to understand the molecular mechanism of signal tran
 sduction in the G-protein coupled receptor rhodopsin. In 2004\, she joined
  The Rockefeller University\, where she continued to develop methods in ex
 pression and purification of membrane proteins. In 2007\, Elsa became an A
 ssistant Professor of Chemistry at Yale. She was promoted to Associate Pro
 fessor of Chemistry in 2012.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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