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SUMMARY:Rewiring gene regulatory networks: Characterizing the effects of T
 F mutations and alternative isoforms
DTSTART:20180628T120000
DTEND:20180628T130000
DTSTAMP:20260603T231458Z
UID:fa1567d7624e5e5ea515a3d45185b5ac89007803fac7df2933cd616a
CATEGORIES:Conferences - Seminars
DESCRIPTION:Sachi Inukai\, PhD\nTranscription factors (TFs) can regulate h
 undreds of target genes through sequence-specific DNA binding to cis-regul
 atory sites. Thus\, changes in TF function\, especially in DNA binding\, c
 an have profound effects on interactions within gene regulatory networks. 
 Advances in sequencing technology have revealed that such TF variants are 
 not rare: healthy individuals\, surprisingly\, can carry dozens of coding 
 mutations in TF DNA binding domains (DBDs)\, and ~40% of currently annotat
 ed TF alternative isoforms demonstrate altered DBD sequences compared with
  those of reference forms. It is unclear which of these variants actually 
 alter DNA binding. To better understand how TF mutations affect DNA bindin
 g\, we have characterized dozens of TF variants in vitro using Protein Bin
 ding Microarrays in an attempt to develop a classifier geared specifically
  to predict the effects of mutations on TF–DNA binding. Separately\, we 
 are characterizing TF isoforms by HT-SELEX to understand how alternative s
 plicing may be a cellular strategy to regulate TFs’ DNA-binding function
 .\n 
LOCATION:AI 1153 https://plan.epfl.ch/?room==AI%201153
STATUS:CONFIRMED
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