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SUMMARY:Super-Resolution Microscopy with DNA Molecules
DTSTART:20180822T100000
DTEND:20180822T110000
DTSTAMP:20260407T042105Z
UID:454c54e8a339de547581520a50542552db1511433f6cd7f9092410aa
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Ralf Jungmann\, Max Planck Institute of Biochemistry and
  Ludwig-Maximilians-Universität München (D)\nBIOENGINEERING SEMINAR\n\nA
 bstract:\nSuper-resolution fluorescence microscopy is a powerful tool for 
 biological research. We use the transient binding of short fluorescently l
 abeled oligonucleotides (DNA-PAINT) for simple and easy-to-implement multi
 plexed super-resolution imaging that technically achieves sub-5-nm spatial
  resolution. However\, this does not translate to achievable image resolut
 ion in biological specimen\, mainly due to the lack of readily available s
 mall and specific affinity reagents.\n\nWe introduce Slow Off-rate Modifie
 d Aptamers (SOMAmers) for DNA-PAINT super-resolution microscopy as efficie
 nt and quantitative labeling reagents. We demonstrate the achievable image
  resolution and specificity by labeling and imaging of transmembrane as we
 ll as intracellular targets in fixed and live cell-specimen. \n\nApart fr
 om ever increasing spatial resolution\, efficient multiplexing strategies 
 for the simultaneous detection of hundreds of molecular species are still 
 elusive. We now introduce an entirely new approach to multiplexed super-re
 solution microscopy by designing the blinking behavior of targets with eng
 ineered binding frequency and duration in DNA-PAINT. We assay this kinetic
  barcoding approach in silico and in vitro using DNA origami structures\, 
 show the applicability for multiplexed RNA and protein detection in cells 
 and finally experimentally demonstrate 124-plex super-resolution imaging w
 ithin minutes.\n\nBio:\nSince 2017        Allen Distinguished Inve
 stigator in Epigenetics\nSince 8/2016      Professor for Experimental
  Physics at the Ludwig-Maximilians-Universität München (D)\nSince 12/201
 4    Independent group leader at the Max Planck Institute of Biochemis
 try and Ludwig-Maximilians-Universität München (D)\n2011 - 2014    
     Postdoctoral researcher at the Wyss Institute for Biologically Ins
 pired Engineering at Harvard University (USA) in the groups of Prof. Dr. P
 eng Yin and Prof. Dr. William M Shih\n2010 - 2011        Postdocto
 ral researcher at Technische Universität München (D) in the group of Pro
 f. Dr. Friedrich C. Simmel\n2007 - 2010        Ph.D. with Prof. Dr
 . Friedrich C. Simmel at Technische Universität München (D)\n2005 - 2006
         Diploma Thesis work with Prof. Dr. Paul K Hansma at Univer
 sity of California Santa Barbara (USA)\n2001 - 2006        Studies
  in physics at Saarland University (D)\n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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