BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:BMI SEMINAR // Takeshi Sakaba - Biophysics of transmitter release 
 at the mossy fiber synapse
DTSTART:20190306T121500
DTEND:20190306T131500
DTSTAMP:20260916T044051Z
UID:4fbc19dd450fd8dcd40e4fdd9f1dc86fe1982905e64e6d3bfd9f832f
CATEGORIES:Conferences - Seminars
DESCRIPTION:Takeshi Sakaba\, Laboratory of Molecular Synaptic Function\, G
 raduate School of Brain Science\, Doshisha University\, Kyoto\, Japan\nHip
 pocampal mossy fiber synapses are an important element in the hippocampus 
 and exhibit various forms of synaptic plasticity. To investigate the mecha
 nism of transmitter release and its modulation\, we used presynaptic capac
 itance measurements and EPSC recording to measure the kinetics of transmit
 ter release. In addition\, total internal reflection fluorescence (TIRF) m
 icroscopy was used to visualize single synaptic vesicle dynamics near the 
 plasma membrane. Readily releasable synaptic vesicles are mediated by fusi
 on of already-tethered vesicles in the TIRF field. Vesicle replenishment h
 ad fast and slow phases\, and the fast phase depends on synaptic vesicle p
 riming from already-tethered vesicles. cAMP\, a molecule crucial for LTP\,
  mainly increases the vesicular release probability\, likely by changing t
 he coupling between Ca2+ channels and synaptic vesicles. We compared these
  findings with those obtained at the calyx of Held synapse\, one of the mo
 del synapses in the mammalian CNS\, and discussed similarities and differe
 nces between two synapse types.\n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
END:VEVENT
END:VCALENDAR