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SUMMARY:Use of the Crooks Fluctuation Theorem to Study the Inter-domain Fo
 lding Cooperativity of a Protein
DTSTART:20181008T161500
DTEND:20181008T171500
DTSTAMP:20260506T002536Z
UID:5a7b7ce0242ec72263219a4f14837bfbd6a5675cd4b84f336be275f9
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Carlos Bustamante\, University of California\, Berkeley\
 nThe structures of proteins show a modular architecture comprised of discr
 ete domains. Inter-domain communication regulates function and folding\, w
 hile lack of coupling can lead to protein misfolding\, aggregation and ass
 ociated pathologies. However\, how different regions communicate is still 
 poorly understood. \nUsing single-molecule optical tweezers\, we induce t
 he unfolding of regions of T4 lysozyme and monitor the effect on other reg
 ions not directly acted on. We determine the free energy landscape during 
 mechanical unfolding using Crooks' fluctuation theorem\, and show that the
  order of structural elements along the sequence determines what parts of 
 the folding/unfolding landscape are explored. We speculate that proteins m
 ay have evolved to select certain topologies that increase coupling betwee
 n regions to avoid areas of the landscape that lead to misfolding.\n 
LOCATION:CM 1 2 https://plan.epfl.ch/?room==CM%201%202
STATUS:CONFIRMED
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