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SUMMARY:From Single-Molecules to Single-Cells: Imaging RNA Molecules and R
 NP Complexes
DTSTART:20181019T130000
DTSTAMP:20260413T111223Z
UID:4a2350a9e4f2c991bbd8c727e6795cd5d720366783a2c77384814c5b
CATEGORIES:Conferences - Seminars
DESCRIPTION:Professor David Rueda \, FRSC Chair\, Molecular and Cellular 
 Medicine\, Molecular Virology\, Department of Medicine Head\, Single Molec
 ule Imaging Group\, MRC London Institute of Medical Sciences\, Imperial Co
 llege London\, UK\nUnderstanding how nucleic acids and proteins interact 
 to regulate key cellular processes requires the ability to observe these 
 interactions directly. The dynamic nature of many nucleic acid-protein in
 teractions makes it challenging to study them with traditional bulk metho
 ds. Biochemical\, molecular or cellular biology approaches yield ensemble
 - or population-averaged results\, which may conceal key short-lived or 
 low populated intermediates on the reaction pathway. To overcome the aver
 aging problem\, our group develops and applies single-molecule microscopy
  (SMM) approaches to monitor such interactions in real-time. SMM has bec
 ome increasingly important in studies of nucleic acid-protein interactions
  because these techniques provide access to crucial information on how 
 individual molecules or complexes behave in bulk solution and in live cell
 s\, revealing the underlying structural dynamics and heterogeneity in the
  system.  We will present our data investigating some of these interact
 ions on a specific model enzyme that plays key role in essential cellula
 r processes and biotechnology: CRISPR-Cas9. We will also present our rece
 nt efforts to image RNA molecules in live and fixed mammalian cells with 
 fluorgenic RNA aptamers (Mango).\n 
LOCATION:BSP 407 https://plan.epfl.ch/?dim_floor=4&lang=fr&dim_lang=fr&tre
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STATUS:CONFIRMED
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