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SUMMARY:Using a Nanopore to Watch Enzymes at Work
DTSTART:20181105T121500
DTSTAMP:20260609T140943Z
UID:5c1f530d97afbc56403db617a28e2190014d2645cdb46ab4831913dc
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Jens H. Gundlach\, Department of Physics\, University of
  Washington\, Seattle\, Washington (USA)\nDISTINGUISHED LECTURE IN BIOLOGI
 CAL ENGINEERING\n(sandwiches served)\n\nAbstract: \nWe have engineered a h
 igh-resolution molecular sensor based on an ion current flowing through th
 e protein nanopore MspA. This sensor has enabled nanopore sequencing of DN
 A. Here\, I will show how we have further adapted this nanopore technology
  as a single-molecule tool called SPRNT (Single molecule Picometer Resolut
 ion Nanopore Tweezers). SPRNT permits real-time observation of enzyme mech
 anics with unprecedented spatio-temporal resolution while it simultaneousl
 y reveals the nucleotide sequence within the enzyme. I will show hereto un
 seen detail in the motion of several different enzymes along nucleic acids
 . In the helicase Hel308\, we observe two steps\, an ATP-dependent and an 
 ATP-independent step\, for each full nucleotide progression by the enzyme.
  The stepping kinetics depends on the sequence of the DNA substrate. In ad
 dition\, using the core polymerase of the transcription complex (RNAP)\, w
 e observe detailed enzyme activity at sequence-specific pausing sites.\n 
 \nBio:\nPhysik-Vordiplom \, Johannes Gutenberg Universit¨at\, Mainz\, Ger
 many\, 1982.\nPhysik-Diplom \, Johannes Gutenberg Universit¨at\, Mainz\, 
 Germany\, 1986.\nPh.D. (Nuclear Physics)\, University of Washington\, 1990
 .\nPostdoctoral Research Associate (Experimental Gravitation)\,\nUniversit
 y of Washington\, 1990-93.\nResearch Assistant Professor\, University of W
 ashington\, 1993-98.\nResearch Associate Professor\, University of Washing
 ton\, 1998-2004.\nFull Professor\, University of Washington\, 2004-present
 .
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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