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SUMMARY:Functional interplay between chromatin organizers and condensins f
 or the control of chromosome conformation and segregation in bacteria
DTSTART:20181211T121500
DTSTAMP:20260501T144151Z
UID:9c8e3574873ed9eadc74347e2d5bab8733469980295ef2a525a5cb60
CATEGORIES:Conferences - Seminars
DESCRIPTION:Frédéric Boccard\, Institut de Biologie Intégrative de la C
 ellule\, CNRS\, Gif-sur-Yvette\, France\nCondensation of DNA molecules res
 ults in the formation of chromosomes. In bacteria\, the chromosome is a fo
 lded structure called nucleoid. The objectives of our laboratory are to re
 veal the principles of chromosome organization\, characterize the molecula
 r mechanisms involved\, and analyze the coordination of chromosome segrega
 tion with progression of the cell cycle in different bacterial models. Usi
 ng Chromosome-Conformation-Capture methods combined with genomics\, fluore
 scence microscopy and genetic approaches\, we have disclosed in E. coli an
 d Pseudomonas aeruginosa the three-dimensional folding of the chromosome a
 nd characterized the activity of several factors involved in chromatin org
 anization and nucleoid conformation. At short scale\, the contact map reve
 aled the presence of domains ranging in size from 50kb to 300kb with long 
 and highly expressed genes frequently found between domains. At large scal
 e\, long distance contacts accounted for the presence of macrodomains. By 
 analyzing the contact frequency in several mutants\, we revealed the modus
  operandi of chromatin organizers and condensins as well as their interpla
 y in controlling short- and long-range DNA contacts.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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