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SUMMARY:Seeing the Forest and the Trees: Single Nucleus Sequencing in the 
 Spinal Cord
DTSTART:20190118T110000
DTEND:20190118T120000
DTSTAMP:20260408T083834Z
UID:734530625982c144dba47121178b69cbe749384a8de1111010d2ca96
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr Ariel Levine\, NIH Neurological Institute NINDS\, USA.\nTo 
 understand the cellular basis of behavior\, it is necessary to know the ce
 ll types that exist in the nervous system and their contributions to funct
 ion. Spinal networks are essential for sensory processing and motor behavi
 or and provide a powerful system for identifying the cellular correlates o
 f behavior. We sought to leverage the recent revolution in single cell tra
 nscriptional profiling to reveal the cell types of the adult mouse spinal 
 cord and their activity. First\, we adapted a robust strategy for massivel
 y parallel single nucleus RNA sequencing that can be used for complex cent
 ral nervous system tissue without driving experimental changes in gene exp
 ression. Next\, we created a molecular and cellular atlas of the adult mou
 se spinal cord. We identified and characterized forty-three neuronal popul
 ations that encompass dorsal\, ventral\, excitatory\, and inhibitory cell 
 types and include both previously known and novel populations. Finally\, w
 e used the dynamic transcriptional signature of neuronal activity to “ma
 p” activated neurons associated with a sensory response and a motor beha
 vior. This approach can now be used to link single cell resolution to syst
 em wide changes and reveal gene the dynamic biological responses to behavi
 or\, injury\, and disease.\n\nBio\nDr. Levine received an undergraduate de
 gree in biology from Brandeis University in 2000\, a Ph.D. from The Rockef
 eller University in 2008\, and an M.D. from Cornell University in 2009. Du
 ring her postdoctoral research with Dr. Samuel Pfaff at The Salk Institute
 \, she identified a novel population of spinal neurons that encode “moto
 r synergies” – modular neural programs for simple movements that are t
 hought to underlie a wide variety of common behaviors. She joined the Nati
 onal Institutes of Health in 2015 where her lab studies how the molecules\
 , neurons\, and circuits of the spinal cord mediate normal behavior and le
 arn.
LOCATION:Campus Biotech H8 Auditorium D https://plan.epfl.ch/?room==H8%201
 %20144.167
STATUS:CONFIRMED
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