BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Memento EPFL// BEGIN:VEVENT SUMMARY:Bringing Orthogonality in the Tetrazine Ligation with (strained) a lkenes DTSTART:20190528T161500 DTSTAMP:20260428T021456Z UID:baa1bc90b1bcc9631c5bd8ba53f8a8ff6655575ae3ea2de4321d9a70 CATEGORIES:Conferences - Seminars DESCRIPTION:Dr. Kimberley Bonger\, Radboud University\, Netherlands\nThe d evelopment of bioorthogonal reactions made it possible to visualize and st udy biomolecules in their native cellular context and contributed to advan ced targeted drug delivery strategies. Bonger's research group is interest ed in developing novel bioorthogonal chemistry and apply this in the targe ting of specific cell types. They recently added non-strained\, highly sol uble and stable vinylboronic acids (VBA) as reactants to the bioorthogonal toolbox which react with tetrazines in an inverse-electron demand Diels-A lder reaction. They have observed exceptional high reaction rates between non-strained vinylboronic acids (VBAs) and dipyridyl tetrazines relative t o that of tetrazines lacking such dative coordinating ligand. As VBAs are mild Lewis acids\, they hypothesize that coordination of the pyridyl to th e boronic acid promotes the tetrazine ligation. In the current presentatio n\, they explore the scope and molecular origins of the observed VBA react ivity in more detail and benefit from its unique properties in the simulta neous orthogonal tetrazine labelling of proteins. In addition\, they furth er extended the VBA toolbox as chemically-triggered cleavable linkers for targeted drug delivery with the specific focus on autoreactive B-cells.\n  \nReferences:\n1) Selma Eising\, Francis Lelivelt\, Kimberly M. Bonger*. Vinylboronic Acids as Fast Reacting\, Synthetically Accessible\, and Stab le Bioorthogonal Reactants in the Carboni–Lindsey Reaction. Angew. Chem. Int. Ed. 2016\, 55\, 12243. \n2) Selma Eising\, Nicole van der Linden\, F leur Kleinpenning\, Kimberly M. Bonger. Vinylboronic Acids as Efficient Bi oorthogonal Reactants for Tetrazine Labeling in Living Cells. Bioconjug. C hem. 2018\, 29\, 982.\n3) Selma Eising\, Bo-Tao Xin\, Fleur Kleinpenning\, Jurriaan J. A. Heming\, Bogdan I. Florea\, Herman S. Overkleeft and Kimbe rly M. Bonger* Coordination-Assisted Bioorthogonal Chemistry: Orthogonal T etrazine Ligation with Vinylboronic Acid and a Strained Alkene\, ChemBioCh em 2018\, 15\, 1648.\n\nBiosketch :\nKimberly Bonger obtained her M.Sc deg ree in Organic Chemistry from the Free University in Amsterdam in 2002. In 2008 she received her PhD from Leiden University working under the superv ision of Prof. Dr. Gijs van der Marel and Prof. Dr. Hermen Overkleeft on t he design and synthesis of dimeric ligands for G-protein coupled receptors involved in human reproduction. After spending almost four years as a pos tdoc at Stanford University in the laboratory of Thomas Wandless working o n molecular tools to control protein stability\, she returned to the Nethe rlands as an assistant professor in Chemical Biology at the Radboud Univer sity in Nijmegen. Her research focusses on the development of novel bioort hogonal reactions and targeted drug delivery strategies as well as the fun damental understanding of cellular mechanisms involved in autoimmunity.\n   LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 STATUS:CONFIRMED END:VEVENT END:VCALENDAR