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SUMMARY:Focusing on mitochondria with super-resolution microscopy
DTSTART;VALUE=DATE:20120611
DTSTAMP:20260509T131747Z
UID:8f7ce63ece8df78e37a4f4cbdc707f28fa3c7eddd4c5aa224fcf0229
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Stefan Jakobs\, Max Planck Institute for Biophysical Che
 mistry\nMitochondria are essential organelles in all eukaryotic cells. The
 ir dysfunction is related to numerous diseases including several devastati
 ng neurodegenerative diseases. The nanoscale distributions of proteins wit
 hin mitochondria are largely unexplored\, due to the small dimensions of t
 he mitochondria and the high protein packaging densities in the mitochondr
 ial membranes. The first part of the talk will report on our recent studie
 s on sub-mitochondrial protein distributions and on the heterogeneity of m
 itochondria within and between cells using STED nanoscopy in combination w
 ith quantitative image analysis. In the second part of the talk I will dis
 cuss our recent progress in the design and application of reversibly switc
 hable fluorescent proteins (RSFPs). RSFPs can be repeatedly photoswitched 
 between fluorescent and non-fluorescent states by irradiation with distinc
 t light wavelengths\, a property that can be exploited to overcome the dif
 fraction barrier in live cell imaging utilizing low light intensities. Pre
 vious RSFPs allowed only a limited number of switching cycles before photo
 destruction. We have recently generated novel RSFPs exhibiting strong resi
 stance against photobleaching and switching fatigue. They are therefore we
 ll suited for reversible saturable optical (fluorescence) transition (RESO
 LFT) nanoscopy enabling the recording of raw data images from living cells
  and tissues with low levels of light.
LOCATION:SV 1717A
STATUS:CONFIRMED
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