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SUMMARY:BioE COLLOQUIA SERIES:  "Biological Imaging at High Spatiotemporal
  Resolution"
DTSTART:20191007T121500
DTSTAMP:20260916T042519Z
UID:a8907d4564589e1676af14adf59fb4d141620a6f1a4649fe969ec9e8
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Hari Shroff\, National Institute of Biomedical Imaging a
 nd Bioengineering (NIBIB-NIH)\, Bethesda\, MD (USA)\nWEEKLY BIOENGINEERING
  COLLOQUIA SERIES\n(sandwiches served)\n\nAbstract: \nI will discuss our e
 fforts to improve structured illumination microscopy (SIM) and light-sheet
  microscopy. SIM doubles the spatial resolution of light microscopy\, requ
 iring lower light intensities and acquisition times than other super-resol
 ution techniques. I will present SIM implementations that enable resolutio
 n doubling in live volumes > 10-20x thicker than possible with conventiona
 l SIM\, as well as hardware modifications that enable effectively ‘insta
 nt’ SIM imaging at rates 10-100x faster than other SIM. Newer applicatio
 ns of instant SIM\, including combination with total internal reflection (
 TIRF) and with adaptive optics will also be discussed. \nThe second half 
 of the talk will focus on the development of dual-view inverted selective 
 plane illumination microscopy (diSPIM)\, and subsequent application to the
  noninvasive study of neurodevelopment in nematodes. Newer multiview resul
 ts with more objectives and more views\, further improving spatial resolut
 ion\, will also be shown. Applications of these technologies will be prese
 nted\, including computational methods for untwisting worm embryos\, and c
 alcium and behavioral imaging of moving embryos. Finally\, unpublished met
 hods for rapidly processing very large\, TB-scale light-sheet datasets wil
 l be presented. \n\n\nBio:\nDr. Hari Shroff received a B.S.E. in bioengin
 eering from the University of Washington in 2001\, and under the supervisi
 on of Dr. Jan Liphardt\, completed his Ph.D. in biophysics at the Universi
 ty of California at Berkeley in 2006 . He spent the next three years perfo
 rming postdoctoral research under the mentorship of Eric Betzig at the How
 ard Hughes Medical Institute's Janelia Farm Research Campus where his rese
 arch focused on development of photactivated localization microscopy (PALM
 )\, an optical superresolution technique. Dr. Shroff is now chief of NIBIB
 's Section on High Resolution Optical Imaging laboratory\, where he and hi
 s staff are developing new imaging tools for application in biological and
  clinical research.\n\nZoom link for attending remotely:  https://epfl.zo
 om.us/j/118303710\n \n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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