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SUMMARY:Conformational Dynamics and Diversity of Single G Protein-Coupled 
 Receptors Trapped in Solution or Imaged in Living Cells
DTSTART:20111010T070000
DTSTAMP:20260921T202808Z
UID:34826a0d929cdfb16df24a14b8d4dda386a4ad20b1caf81593d64241
CATEGORIES:Conferences - Seminars
DESCRIPTION: Alexandre Fürstenberg\, University of Geneva\nWe focus on ou
 r use of an Anti-Brownian ELectrokinetic (ABEL) trap\, a novel device enab
 ling the study of individual fluorescent nanoscale objects in solution wit
 hout requiring chemical immobilization\, to investigate the conformational
  dynamics of the b2-adrenergic receptor (b2AR)\, a canonical GPCR involved
  in smooth muscle regulation. The ABEL trap uses fast electrokinetic feedb
 ack in a microfluidic configuration to enable direct observation of single
  fluorescently-labelled β2AR molecules for hundreds of milliseconds to se
 conds. By using a conformation-sensitive reporter dye\, we observe a diver
 sity of fluorescence intensity and lifetime levels in individual β2AR mol
 ecules\, indicating changes in radiative lifetime and the existence of a v
 ast range of discrete conformational states. We find that agonist binding 
 increases the dwell times of these states and monitor millisecond fluctuat
 ions within these states.
LOCATION:BM5204 
STATUS:CONFIRMED
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