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SUMMARY:Increasing the throughput in FIB/SEM tomography and targeted cryo-
 lamella preparation
DTSTART:20190329T140000
DTSTAMP:20260510T164909Z
UID:5f8c1134f671d5ccc6062a7af787ba9d53566f25cd171609d9869220
CATEGORIES:Conferences - Seminars
DESCRIPTION:Professor Alex de Marco\, Monash University\, Melbourne\, Aust
 ralia http://www.demarco-lab.com/research\nFocused Ion Beam (FIB) milling 
 is a key technology for nano-scale cellular imaging. The utility of FIB ra
 nges from large volume imaging of resin embedded samples to cryo-lamella p
 reparation of frozen-hydrated samples\, which make this technology indispe
 nsable for modern imaging requirements. Our work demonstrates the advantag
 es of working with non-conventional plasma FIB sources (especially the use
  of reactive species such as oxygen) for fast FIB/SEM tomography and cryo-
 lamella preparation\, demonstrating a throughput increase up to 30% and su
 perior sample compatibility. In association with the use of optimised beam
 s we developed an integrated correlative light and FIB/SEM setup which rem
 oves the need of cryo-transfers between cryo-light microscopy and cryo-lam
 ella preparation. This setup simplifies the procedure for correlative imag
 ing\, it allows better targeted cryo-lamella preparation and reduces the i
 ce contamination which typically curses cryo-samples.\n\nBio :\nI obtained
  my PhD at EMBL working in the group of John Briggs. Here\, I contributed 
 to the development of image processing for high resolution sub-tomogram av
 eraging while investigating the structural changes occurring during the vi
 ral maturation of HIV-1. After my PhD I moved to FEI Company (now part of 
 ThermoFisher Scientific) where I worked as product manager for FIB/SEM mic
 roscopes and correlative microscopy. At the end of 2015 I established my l
 aboratory at Monash University. Here\, our main focus is on methods and ha
 rdware development for cryo-EM and correlative FIB/SEM microscopy.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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