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SUMMARY:Buckling of epithelium growing under spherical confinement
DTSTART:20190409T121500
DTSTAMP:20260502T050215Z
UID:961629a3f3c82b1fd45036c522a6882deeb3ccbe2fcc74594d225a56
CATEGORIES:Conferences - Seminars
DESCRIPTION:Aurélien Roux\, Biochemistry Department\, UNIGE\nMany organs\
 , such as the gut or the spine are formed through folding of an epithelium
 . While genetic regulations of cell fates leading to epithelium folding ha
 ve been investigated\, mechanisms by which forces sufficient to deform the
  epithelium are generated are less studied. Here we show that cells formin
 g an epithelium onto the inner surface of spherical elastic shells protrud
 e inward while growing. By measuring the pressure and local forces applied
  onto the elastic shell\, we show that this folding is induced by compress
 ive stresses arising within the epithelial layer: while growing under sphe
 rical confinement\, epithelial cells are subjected to lateral compression\
 , which induces epithelium buckling. While several fold initiations can be
  observed within one capsule\, final shapes often show one or two folds. W
 hile analytical theory of epithelium buckling predicted a single fold at e
 quilibrium\, multicellular simulations showed several folds occurred from 
 a competition between epithelium bending\, growth and adhesion to the shel
 l. By quantitatively comparing the shapes of buckled epithelium predicted 
 by theory and simulations\, with experimental shapes\, we determined how e
 pithelium bending rigidity\, adhesion and proliferation control buckling\,
  and extracted their values in our experimental conditions. As proposed fo
 r gastrulation or neurulation\, our study shows that forces arising from e
 pithelium proliferation are sufficient to drive epithelium folding.\n\nAna
 stasiya Trushko1\, Ilaria di Meglio1\, Aziza Merzouki2\, Carles Blanch-Mer
 cader1\,3\, Shada Abuhattum4\, Jochen Guck4\, Kevin Alessandri5\, Pierre N
 assoy5\, Karsten Kruse1\,3\,6\, Bastien Chopard2\, Aurélien Roux1\,6\n \
 n1department of Biochemistry\, University of Geneva\, CH-1211 Geneva\, Swi
 tzerland.\n2department of Computer Science\, University of Geneva\, CH-121
 1 Geneva\, Switzerland.\n3department of Theoretical Physics\, University o
 f Geneva\, CH-1211 Geneva\, Switzerland.\n4Biotechnology Center\, Technisc
 he Universität Dresden\, DE-01062\, Dresden\, Germany.\n5Université de B
 ordeaux\, Laboratoire Photonique Numérique et Nanosciences & CNRS and Ins
 titut d'Optique\, LP2N\, UMR 5298\, F-33400\, Talence\, France.\n6National
  Center of Competence in Research Chemical Biology\, University of Geneva\
 , CH-1211 Geneva\, Switzerland.\n\n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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