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VERSION:2.0
PRODID:-//Memento EPFL//
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SUMMARY:Molecular Resolution in Optical Nanoscopy by Breaking the Informat
 ion Barrier
DTSTART:20190528T160000
DTEND:20190528T170000
DTSTAMP:20260510T022724Z
UID:2cfa78814cd388144b51918a4655920dcfc6f3abf9ebbf3cfab66595
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Francisco Balzarotti\nSuper-resolution microscopy methods 
 such as STED and PALM/STORM have revolutionized far-field optical fluoresc
 ence microscopy by going beyond the diffraction limit of light and offerin
 g potentially unlimited resolution. In practice\, however\, the resolution
  of an image is limited by the finite photon budget of fluorescent probes\
 , while their finite emission rate imposes a spatio-temporal trade-off in 
 tracking applications. \n \nBy synergistically combining the strengths o
 f both super-resolution families\, the recently introduced MINFLUX concep
 t tackles these limitations by rendering each emitted photon more informat
 ive. MINFLUX localizes an emitter by repeatedly probing its location with 
 an excitation beam that features a zero of intensity. The emitter position
  is obtained from the knowledge of the beam shape and the number of photon
 s collected at each location of the beam. When compared to conventional ce
 ntroid-localization techniques\, it is possible to reach a given precision
  by using fewer photons\, or conversely\, have an improved precision for t
 he same photon budget.\n \nIn this seminar\, I will present the foundatio
 ns of super resolution optical microscopy and build up towards how MINFLUX
  works. I will discuss published results in nanoscopy (~1 nm resolution) a
 nd single molecule tracking and show recent extensions of the technique fo
 r multicolor three-dimensional operation within large fields of view for f
 ixed and living cells.\n 
LOCATION:BSP 407 https://plan.epfl.ch/?room==BSP%20407
STATUS:CONFIRMED
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