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SUMMARY:Unveiling the dynamic nature of cellular nanopores in living endot
 helial cells by video-rate super-resolution microscopy
DTSTART:20190529T134500
DTEND:20190529T141500
DTSTAMP:20260429T200013Z
UID:ff20d120d99a5b9254660fc4b42c407da9da8aa9f355b2c5e57608ba
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Thomas Huser\nSuper-resolved structured illumination mic
 roscopy (SR-SIM) is among the most flexible\, fastest and least perturbing
  fluorescence microscopy techniques capable of surpassing the optical diff
 raction limit. Current custom-built instruments are easily able to deliver
  two-fold resolution enhancement at video-rate frame rates\, but the cost 
 of the instruments is still relatively high and the physical size of the i
 nstruments is still prohibitively large. Here\, I will present our latest 
 efforts towards realizing a new generation of compact\, cost-efficient and
  high-speed SR-SIM instruments. Tight integration of the structured illumi
 nation microscope capable of video-rate image acquisition with instant ima
 ge reconstruction enables us to realize a super-resolving fluorescence mic
 roscope with the look-and-feel of regular wide-field microscopy. I will de
 monstrate this by presenting dynamics of intracellular transport and movem
 ent in living cells\, in particular the dynamics of liver cell fenestratio
 ns. These nano-sized pores in liver endothelial cells play a particularly 
 crucial role in human physiology\, which is reduced or lost during disease
  and/or aging. To best address these issues from all perspectives\, we uti
 lize a suite of multimodal methods\, e.g. the combination of optical tweez
 ers with optical nanoscopy\, or the combination of temporal and spatial me
 thods of improving the spatial resolution and select the best possible met
 hod for each research question. I will present the pros and cons of these 
 methods\, their combination\, and their applications on select biomedical 
 examples.
LOCATION:BSP 231 https://plan.epfl.ch/?room==BSP%20231
STATUS:CONFIRMED
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