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SUMMARY:Thalamo-cortical synaptic network in motor cortex analyzed with la
 rge volume electron microscopy
DTSTART:20190611T150000
DTEND:20190611T160000
DTSTAMP:20260408T144552Z
UID:3acb923bddd754fab1aad97598bbbad4008a76453b93ed3936301903
CATEGORIES:Conferences - Seminars
DESCRIPTION:Yoshiyuki Kubota\, Division of Cerebral Circuitry National In
 stitute for Physiological Sciences Okazaki\, Japan\nIt is well known that 
 the motor cortex receives signals from the basal ganglia and cerebellum vi
 a thalamo-cortical afferents. We investigated how the thalamo-cortical axo
 n terminals participate in the cortical microcircuit in rat frontal cortex
 . The axon arborization pattern across cortical layers varies significantl
 y among thalamocortical afferents from three motor-related thalamic nuclei
 : the ventral medial nucleus (VM) the ventral anterior (VA) and the ventra
 l lateral (VL) thalamic complex which relay motor information from the bas
 al ganglia (VM/VA) and the cerebellum (VL) respectively (2015\, Cereb Cor 
 25: 221-235). We hypothesized that the synaptic connections of VM/VA and V
 L afferents in the cortical microcircuits are different. A viral vector (p
 al-GFP AAV) was injected into each of three motor-related thalamic nuclei.
  Their target structures in the motor cortex was investigated using a corr
 elated light and electron microscopy (CLEM) with a laser confocal microsco
 py and automated tape-collecting ultramicrotomy (ATUM) with scanning elect
 ron microscopy (SEM). To identify the GFP labeled axonal fibers subsequent
 ly at the electron microscopy we stained blood vessels with lectin and cel
 lular nuclei with DAPI and used them as landmarks in cortical tissue secti
 ons. Firstly images were taken with a laser confocal microscopy. Then the 
 tissue sections were embedded in plastic and sectioned with ATUM for SEM o
 bservation. GFP-labeled thalamo-cortical fibers and their target structure
 s were identified in serial electron micrographs and reconstructed three-d
 imensionally. Our preliminary results indicated that the VA fibers mainly 
 targeted dendritic spines of the layer 5 pyramidal cell.
LOCATION:B1 6 272.043 https://plan.epfl.ch/?room=B16272.043
STATUS:CONFIRMED
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