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SUMMARY:Profiling Histone Modifications in Single Cells to Reveal the Dyna
 mics and Control of Blood Cell Differentiation
DTSTART:20190724T150000
DTEND:20190724T160000
DTSTAMP:20260509T170144Z
UID:202e0309e1ff0ec9704b956aeb37a83255ab3461f8066f11b614702f
CATEGORIES:Conferences - Seminars
DESCRIPTION:Jake Yeung\, Ph.D.\, van Oudenaarden group\, Hubrecht Institut
 e\, Utrecht (NL)\nBIOENGINEERING SEMINAR\n\nAbstract:\nHistone modificatio
 ns form an adaptable epigenetic regulatory layer that package the genome i
 nside the nucleus and mediate dynamic transcriptional programs. This packa
 ging can be modulated by a wide spectrum of post-translational histone mod
 ifications\, regulating the expression of associated genes. During cell di
 fferentiation\, the distribution of these histone modifications changes to
  support the new expression state. These dynamics are ideally studied at t
 he single-cell level\, because it is challenging to isolate discrete inter
 mediate states for bulk analysis. We therefore developed a high-throughput
  method that uses chromatin immunocleavage (ChIC) to profile histone modif
 ications in more than 15000 individual cells (scChIC-seq).\n\nIn this talk
 \, I will focus on the challenges of analyzing single-cell histone modific
 ation data to reconstruct differentiation dynamics from single-cell signal
 . In particular\, I will present machine learning strategies that model sp
 arse count data and project onto lower-dimensional manifolds in order to p
 redict relationships between cells as well as relationships across genomic
  regions.\n\nI will also discuss using systems biology methods to infer tr
 anscription factor (TF) activities and how to apply this to single cell da
 ta. This approach infers key TF binding motifs and predicts master regulat
 ors underlying transcriptional programs of hematopoiesis.\n\nFinally\, I w
 ill talk about integrating single-cell datasets of different histone modif
 ications to elucidate coordination of epigenetic reprogramming during bloo
 d cell differentiation. Overall\, scChIC-seq enables the dynamic reconstru
 ction of the global chromatin landscape during in vivo differentiation.\n
  
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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