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SUMMARY:"Exploring dendritic integration in awake mice using simultaneous 
 voltage and calcium imaging and electrophysiology.”
DTSTART:20190723T090000
DTSTAMP:20260408T055759Z
UID:c3e261959d46874961502d70b389424f316171195f8d6b4cb687a741
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr Christopher Roome\nOkinawa Institute of Science and Technol
 ogy\, Okinawa\, Japan \nAbstract\nTo investigate dendritic integration in
  awake mice\, we recently developed a technique to simultaneously record 
 dendritic voltage and calcium signalling and somatic activity with high s
 patio-temporal resolution (Roome and Kuhn 2018). Single cerebellar Purkinj
 e neurons were labelled with voltage sensitive dye ANNINE-6plus and the g
 enetically encoded calcium indicator GCaMP6f\, using a chronic cranial wi
 ndow with access port (Roome and Kuhn\, 2014) and imaged by two-photon mi
 croscopy (line scans at 2 kHz).\nBy recording subthreshold synaptic inputs
  localized to dendritic branchlets\, rapid (1ms) suprathreshold dendritic
  spikes and their corresponding calcium signals\, and somatic output simu
 ltaneously\, we examine how spontaneous and sensory-evoked information pr
 ocessing occurs in Purkinje neuron dendrites of awake mice. Purkinje neuro
 n dendritic integration is far more complex\, dynamic\, and fine scaled t
 han anticipated\, even in resting animals.\n 
LOCATION:SV 2510 https://plan.epfl.ch/?room=SV2510
STATUS:CONFIRMED
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