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SUMMARY:NMR-based tools for structural biology in gels and cells
DTSTART:20190920T111500
DTEND:20190920T123000
DTSTAMP:20260405T180218Z
UID:e6e456226675d22bc21210ef6353044a424709b6feb684233d78ebf8
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Galia Debelouchina\, UCSD - University of California San
  Diego\nRecent advances in structural biology have dramatically expanded t
 he scope of proteins and assemblies that are now amenable to structural an
 alysis. Yet\, many biological systems display dynamics and undergo transfo
 rmations that have been difficult to capture experimentally\, especially i
 n complex or native settings. In this context\, my group has been developi
 ng NMR-based methodologies to describe such challenging systems and to pav
 e the way to the next exciting structural biology frontier\, the cellular 
 environment. Here\, I will present our progress on two fronts: 1) Developm
 ent of NMR methodologies to describe the molecular basis of protein phase 
 transitions from the liquid to the gel and solid states. In particular\, I
  will focus on the transitions displayed by heterochromatin protein 1α (H
 P1α)\, a key player in gene regulation and chromatin organization. 2) Dev
 elopment of sensitivity-enhanced NMR approaches suitable for the cellular 
 environment. Our work is based on a methodology called dynamic nuclear pol
 arization (DNP) which can transfer polarization from electron to nuclear s
 pins and thus increase nuclear signals by several orders of magnitude. For
  this purpose\, we develop small molecule DNP polarization agents that con
 tain unpaired electron spins and that can be targeted selectively to a pro
 tein of interest. These agents have allowed us to obtain NMR structural da
 ta of tiny amounts of proteins (1-5 μg) and to record interesting observa
 tions about the DNP polarization transfer mechanisms in such targeted syst
 ems
LOCATION:BCH 3303 https://plan.epfl.ch/?room==BCH%203303
STATUS:CONFIRMED
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