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SUMMARY:Blue Brain Seminar – Thalamo-cortical synaptic network in motor 
 cortex analyzed with large volume electron microscopy
DTSTART:20190611T150000
DTEND:20190611T160000
DTSTAMP:20260511T081303Z
UID:64c7e49ce97da1eaf0baa4111fad6158a9557b0425271543acc5858e
CATEGORIES:Conferences - Seminars
DESCRIPTION:Yoshiyuki Kubota\nThe EPFL Blue Brain Project is delighted to 
 announce that the next seminar in the series in Neural Computation\, will 
 be on “Thalamo-cortical synaptic network in motor cortex analyzed with l
 arge volume electron microscopy”.  The seminar will be given by Yoshiyu
 ki Kubota from the Division of Cerebral Circuitry National Institute for P
 hysiological Sciences Okazaki\, Japan.\n\nBio: Yoshiyuki Kubota is current
 ly Associate Professor\, Division of Cerebral Circuitry\, National Institu
 te for Physiological Sciences\, Okazaki\, Japan. He gained his Ph.D. in Me
 dical Sciences from Osaka University in Japan and previously worked as a r
 esearcher at RIKEN and as an Assistant Professor at Kagawa Medical School\
 , Takamatsu\, Japan. His postdoctoral research was conducted at the Univer
 sity of British Columbia\, Vancouver\, Canada\; University of Tennessee\, 
 Memphis\, USA and\; JSPS\, Osaka University\, Osaka\, Japan.\n\nAbstract: 
 It is well known that the motor cortex receives signals from the basal gan
 glia and cerebellum via thalamo-cortical afferents. We investigated how th
 e thalamo-cortical axon terminals participate in the cortical microcircuit
  in rat frontal cortex. The axon arborization pattern across cortical laye
 rs varies significantly among thalamocortical afferents from three motor-r
 elated thalamic nuclei: the ventral medial nucleus (VM) the ventral anteri
 or (VA) and the ventral lateral (VL) thalamic complex which relay motor in
 formation from the basal ganglia (VM/VA) and the cerebellum (VL) respectiv
 ely (2015\, Cereb Cor 25: 221-235). We hypothesized that the synaptic conn
 ections of VM/VA and VL afferents in the cortical microcircuits are differ
 ent. A viral vector (pal-GFP AAV) was injected into each of three motor-re
 lated thalamic nuclei. Their target structures in the motor cortex was inv
 estigated using a correlated light and electron microscopy (CLEM) with a l
 aser confocal microscopy and automated tape-collecting ultramicrotomy (ATU
 M) with scanning electron microscopy (SEM). To identify the GFP labeled ax
 onal fibers subsequently at the electron microscopy we stained blood vesse
 ls with lectin and cellular nuclei with DAPI and used them as landmarks in
  cortical tissue sections. Firstly images were taken with a laser confocal
  microscopy. Then the tissue sections were embedded in plastic and section
 ed with ATUM for SEM observation. GFP-labeled thalamo-cortical fibers and 
 their target structures were identified in serial electron micrographs and
  reconstructed three-dimensionally. Our preliminary results indicated that
  the VA fibers mainly targeted dendritic spines of the layer 5 pyramidal c
 ell.\n\nThe seminar is an open event\, at the Blue Brain offices in the Ca
 mpus Biotech\, Geneva.  Upon arrival at the Campus Biotech\, please sign 
 in at the Campus Biotech reception.\n\nTuesday 11 June 2019\n15:00-16:00\n
 \nBlue Brain Project\nCampus Biotech\nChemin des Mines 9\nGeneva\n 
LOCATION:
STATUS:CONFIRMED
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