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VERSION:2.0
PRODID:-//Memento EPFL//
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SUMMARY:ViTest - A rapid field-based diagnostic tool to detect grapevine d
 iseases
DTSTART:20191025T123000
DTEND:20191025T133000
DTSTAMP:20260602T105927Z
UID:c7e2b0cbe208a02612e4c87e281a8d8f2d59ae8f6381976131685a6f
CATEGORIES:Conferences - Seminars
DESCRIPTION:2019 Igem team\nViTest is a fast point-of-care nucleic acid te
 st to differentiate between two grapevine diseases: Flavescence Dorée and
  Bois Noir. Flavescence Dorée is infectious and needs to be quarantined q
 uickly while Bois Noir is not. \n\nWe start by extracting DNA from infect
 ed plant material using a microneedle patch. We then amplify the sequences
  corresponding to grapevine (endogenous control) and the diseases (if they
  are present). This step is performed using Recombinase Polymerase Amplifi
 cation\, an isothermal nucleic acid amplification method. The created ampl
 icons are transcribed into mRNA and bind to a toehold sensor\, thus activa
 ting a reporter gene.\n\nOnce this gene is translated\, it produces catech
 ol 2\,3 dioxygenase which reacts with catechol and creates a colorimetric 
 feedback. Both the transcription of the amplicons and the translation of t
 he reporter gene are done in our homemade OnePot PURE cell-free system.\n\
 n\n \n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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