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SUMMARY:Principles of Xenopus appendage regeneration
DTSTART:20191121T160000
DTEND:20191121T180000
DTSTAMP:20260924T080110Z
UID:d0610db0785d447b0acfb31d1202fdb89b68e052cc3b0566852678ab
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Can Aztekin\, Departement from University of Cambridge\nSe
 minar from Dr. Can Aztekin\, Department from University of Cambridge\nAffi
 liation: The Welcome Trust/Cancer Research UK Gurdon Institute\, Universit
 y of Cambridge\n \nAbstract:\n \nCan Aztekin Principles of Xenopus appen
 dage regeneration Unlike mammals\, Xenopus tadpoles have a high regenerati
 ve potential for their appendages such as tails and limb buds. Furthermore
 \, having naturally occurring regeneration-competent and-incompetent stage
 s allows comparative studies to reveal principles of regeneration that can
  be harnessed for regenerative medicine purposes. However\, regeneration h
 as been poorly characterized at the single-cell level\, and cell types and
  transcriptome changes accompanying regeneration remains largely elusive. 
 To address this\, we first performed scRNA-seq to intact tails and in a ti
 me course following tail amputation in regeneration-competent and -incompe
 tent stage tadpoles (Aztekin C. et al.\, Science\, 2019). By comparing the
  transcriptome of single cells\, we identified a previously uncharacterize
 d population which we termed as regeneration-organizing-cells (ROCs). ROCs
  are present in intact tails and specifically relocalize to the amputation
  plane of regeneration-competent tadpoles and form the specialized wound e
 pidermis. Elimination of ROCs blocks regeneration\, and grafting tissues t
 hat contain ROCs enable ectopic outgrowths. By secreting a cocktail of lig
 ands that can enable progenitor cell proliferation\, ROCs act as a signal 
 centre that mediates regeneration. ROCs relocalization ability depends on 
 the myeloid lineage activity. Upon tail amputation\, regeneration-incompet
 ent tadpoles fail to suppress amputation induced inflammation. Meanwhile\,
  suppression of inflammation enables decreased apoptosis levels which then
  enables remodelling of the extracellular matrix\, and finally ROCs to rel
 ocalize to the amputation plane. As a next step\, we ask whether tail and 
 limb regeneration utilize similar mechanisms and ROCs based regeneration m
 odel is also seen in Xenopus limb regeneration. Collectively\, identificat
 ion of a single cell type\, ROCs\, that can orchestrate regeneration and c
 haracterize the specialized wound epidermis offers new perspectives to reg
 enerative medicine.\n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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