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SUMMARY:BMI Seminar //  Valentina Emiliani - Wave-front shaping and circui
 t optogenetics
DTSTART:20221123T160000
DTEND:20221123T170000
DTSTAMP:20260916T061414Z
UID:f7a427b4f4663b391e3fd0405ca69e9d55dd138d6ee06c3b996dbc8a
CATEGORIES:Conferences - Seminars
DESCRIPTION:Valentina Emiliani\, Wavefront-engineering microscopy group\, 
 Vision Institute\, Paris\, France\nGenetic targeting of neuronal cells wit
 h activity reporters (calcium or voltage indicators) has initiated the par
 adigmatic transition whereby photons have replaced electrons for reading l
 arge-scale brain activities at cellular resolution. In parallel\, optogene
 tics has demonstrated that targeting neuronal cells with photosensitive mi
 crobial opsins\, enables the transduction of photons into electrical curre
 nts of opposite polarities thus writing\, through activation or inhibition
 \, neuronal signals in a non-invasive way.\nThese progresses have in turn 
 stimulated the development of sophisticated optical methods to enable “a
 ll optical” in depth brain circuits interrogation with high spatial and 
 temporal resolution on large volumes.\nHere\, we will review the most sign
 ificant breakthroughs of the past years\, which enable reading and writing
  neuronal activity at the relevant spatiotemporal scale for brain circuits
  manipulation\, with particular emphasis on the most recent advances in wh
 at we named circuit optogenetics: a combination of wave front shaping appr
 oaches\, including holographic light illumination\, temporal focusing\, wi
 th opsins engineering and laser development enabling the control of single
  or multiple targets independently in space and time with single-neuron an
 d single-spike precision\, at large depths.  Finally\, we will show few e
 xamples of all-optical manipulation of neuronal circuits including the inv
 estigation of inter-layers signal propagation in mouse retina circuits and
  the investigation of neuronal connectivity in mice visual cortex.\n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://epfl.zoom.u
 s/j/62550191552?pwd=OFRDVzBHb0cyVG1wYkxUSFFEMUNPdz09
STATUS:CONFIRMED
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