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SUMMARY:EPFL BioE Talks SERIES  "Functional Single-Cell Deep Phenotyping -
  The ‘DropMap’ Platform and Its Use for High-Resolution Immune Monitor
 ing"
DTSTART:20201012T160000
DTEND:20201012T163000
DTSTAMP:20260404T173958Z
UID:d07427a18ea9635ef82df9179aa29d7e1d563c68bedf6a50192238b8
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Klaus Eyer\, ETH Zurich (ZH)\nWEEKLY EPFL BIOE TALKS SER
 IES\n \n(note that this talk is number one of a double-feature seminar - 
 see details of the second talk here) \n\nAbstract:\nIndividual cells are t
 he functional units within any immune response\, and their dynamic frequen
 cies\, functionalities and degrees of activity shape the response and defi
 ne its outcome. Hence\, the quantitative measurement of cellular functiona
 lity with sufficient dynamic and analytical resolution is of upmost import
 ance to understand the induced immune reaction\, to predict its outcome an
 d to evaluate the efficacy of interventions such as vaccination. In order 
 to assess immune functionalities quantitatively\, we have developed a simp
 le microfluidic system\, ‘DropMap’\, in which single cells are compart
 mentalized in tens of thousands of 50-pL droplets\, and analyzed in two-di
 mensional droplet arrays using a fluorescence relocation-based immunoassay
 . Using DropMap\, we characterize the functionality and activity of a rang
 e of different immune cells\, from neutrophils to macrophages\, from T cel
 ls to antibody-secreting cells\, in a variety of induced disturbances. One
  of our research focus lies on vaccination and the characterization\, unde
 rstanding and exploitation of vaccine-mediated protection. Here\, we have 
 specialized on the analysis of individual antibody-secreting cells and the
 ir antibodies\, allowing us to characterize the induced and present antibo
 dy repertoire with single antibody resolution and high analytical resoluti
 on. In this presentation\, I will explain the technology behind DropMap an
 d show some of its applications within our research projects. In particula
 r\, I want to focus on how single-antibody resolution is used to study imm
 unization\; and how such a system can be used to characterize the antibody
  repertoire. In the vision of our laboratory\, deciphering the dynamic pro
 cesses at the single-cell level is critical to describe and understand the
  fundamental mechanisms underlying immunity\, to develop new and improved 
 strategies for vaccination and cancer immunotherapy\, and to diagnose and 
 treat inflammatory\, autoimmune and infectious diseases efficiently.\n\nBi
 o:\nKlaus Eyer finished his studies in Pharmaceutical Sciences at ETH Zuri
 ch in 2010 with the federal diploma and ETH medal. In 2014\, he received h
 is medaled doctoral degree from the laboratory of Prof. Dittrich at the In
 stitute of Organic Chemistry at ETH Zurich. He left Switzerland thereafter
  and started as a Postdoctoral fellow in the groups of Prof. Bibette and P
 rof. Griffiths at the ESPCI in Paris. In 2016\, he was awarded a Branco-We
 iss-Fellowship\, and continued as a junior group leader at ESPCI and assoc
 iated researcher at Institute Pasteur in Paris. He received an ERC startin
 g-grant in 2018\, and started his current position as a non-tenure track a
 ssistant professor at the Institute of Pharmaceutical Sciences\, ETH Züri
 ch\, in August 2019. His group focuses on developing novel analytical stra
 tegies to understand the functionalities of individual cells in the contex
 t of complex cellular systems\, such as the immune system and its response
 s in health and disease. The research of his group currently focuses on ga
 thering knowledge to advance the understanding of vaccine-mediated protect
 ion and finding novel diagnostic\, prognostic\, and therapeutic strategies
  in various immune-related diseases and syndromes.\n\n\nZoom link (with re
 gistration) for attending remotely: https://go.epfl.ch/EPFLBioETalks\n\n\n
 IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 
 situation\, this seminar can be followed in the room by at most 23 people\
 , who must wear a face mask at all times.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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