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SUMMARY:Integrative dynamic structural biology of biomolecular assemblies 
 resolved by multi-scale super-resolution FRET spectroscopy and imaging
DTSTART:20201124T171500
DTEND:20201124T181500
DTSTAMP:20260307T131435Z
UID:4dda490fc00ba48f5c5ced7a9d7ddd8f6097dabb0babff6a3f7eab3e
CATEGORIES:Conferences - Seminars
DESCRIPTION:Claus A.M. Seidel (Heinrich Heine University\, Germany)\nFRET 
 spectroscopy and imaging can provide state-specific information on the str
 ucture and dynamics of complex dynamic biomolecular assemblies under ambie
 nt conditions with nanosecond time resolution and single-molecule sensitiv
 ity. To overcome the sparsity of FRET experiments\, we developed procedure
 s to combine these with computer simulations to map biomolecular dynamics 
 and to resolve quantitative integrative structure models at a precision an
 d accuracy better than 3 Å. The integrative structure models are deposite
 d in the new protein data bank\, PDB-dev [1-3]. Moreover\, we combined sup
 er-resolution microscopy via stimulated emission depletion (STED) and Mult
 i-parameter Fluorescence Image Spectroscopy (MFIS) [4] to reach molecular 
 resolution with sub-nanometer precision in molecular imaging of biomolecul
 es and their complexes. While STED-MFIS captures the spatial and temporal 
 information of the cellular context with a resolution below 10 nm\, the co
 ncurrent measurement of Förster resonance energy transfer (FRET) between 
 an excited donor and acceptor provides a zoom with Ångström precision. T
 hus\, integrative super-resolution FRET image spectroscopy exploits these 
 synergies to reach molecular resolution.\nI will introduce the concepts of
  our novel optical tools and demonstrate recent applications: (1) Detectio
 n of a so far hidden functionally important conformational state in the en
 zyme T4 Lysozyme [5]\, (2) Resolving the conformational transitions of Gua
 nylate binding proteins (GBPs) during GTP-controlled phase transition to e
 xert their function as part of the innate immune system of mammalian cells
  [6]. (3) Mapping the dynamic exchange network in chromatin fibers by stud
 ying a 12-mer nucleosome array [7].\n\n[1] Kalinin et al.\; Nat. Methods 9
 \, 1218-1225 (2012).\n[2] Dimura et al.\; Curr. Opin. Struct. Biol. 40\, 1
 63–185 (2016).\n[3] Dimura et. al. Nat Commun. 11\, e5394 (2020).\n[4] W
 eidtkamp-Peters et al.\; Photochem. Photobiol. Sci. 8\, 470-480 (2009).\n[
 5] Sanabria et. al. Nat Commun. 11\, e1231 (2020).\n[6] Kravets et. al.\; 
 eLife 5\, e11479 (2016).\n[7] Kilic et al.\; Nat. Commun. 9\, 235 (2018).\
 n 
LOCATION:https://epfl.zoom.us/j/81648589177
STATUS:CONFIRMED
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