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SUMMARY:Super-resolution by Structured Illumination
DTSTART:20120912T091500
DTSTAMP:20260501T211110Z
UID:aba478af4f1172b4f4b10098f9656175a8590662789abea8b783849a
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Hesper Rego\, Harvard School of Public Health\nStructured-
 illumination Microscopy (SIM) is a super-resolution technique that relies 
 on patterned illumination to move high-resolution information into the nor
 mal pass-band of a fluorescence microscope. SIM can double the resolution 
 of a light microscope using conventional fluorescence\, and can achieve th
 eoretically unlimited resolution if a nonlinear fluorescence phenomenon is
  exploited. Here I will briefly discuss the theory and practical design co
 nsiderations of SIM.  I will then show examples of fixed and live-cell SI
 M in two and three dimensions and in multiple colors. Finally\, I will dis
 cuss our work to improve nonlinear SIM such that it is a biologically comp
 atible super-resolution method. Using fluorescence photoswitching\, an inh
 erently nonlinear phenomenon\, we have reached 40-nm resolution with low l
 ight intensities and needing 1000-fold fewer images than localization-base
 d super-resolution methods.
LOCATION:AI 1153 https://plan.epfl.ch/?room==AI%201153
STATUS:CONFIRMED
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