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SUMMARY:Pulling on chromatin: mechanical micro-manipulation of chromatin i
 n live cells
DTSTART:20210322T100000
DTSTAMP:20260507T190742Z
UID:680aa5fbe5464f592342e0fd6414e0aa14d78f33cd773e5b7c32d8fc
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Veer Keizer - Antoine Coulon lab- Institute Curie Paris\nO
 ver the past decades\, our understanding of the physical organization of t
 he genome has improved tremendously. However\, methods to actively probe t
 he relationship between the mechanical properties of chromatin\, its organ
 ization and function in live cells are still lacking. To address this gap\
 , we have developed a novel tool to directly manipulate chromatin in live 
 cells using magnetic forces. By targeting iron-containing nanoparticles to
  a specific genomic locus and applying a controlled magnetic field\, we we
 re able to physically move chromatin through nuclear space for the first t
 ime. We determined the spatial\, temporal and force scales at which chroma
 tin reacts to mechanical perturbations and observed partially reversible a
 nd multi-component viscoelastic properties of chromatin at the time scale 
 of minutes. Moreover\, we were able to assess the interaction with the sur
 rounding chromatin landscape. We are currently building a mechanistic mode
 l that captures our findings. This novel tool opens new avenues to investi
 gate the physical properties of the genome at various scales and relate th
 is to key genome functions including transcription\, DNA damage/repair\, r
 eplication\, chromosome segregation and genome integrity.
LOCATION:https://epfl.zoom.us/j/85236482634?pwd=T3NXamcwa1dSdXV0VWR2M0s2b2
 81UT09
STATUS:CONFIRMED
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