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SUMMARY:Biophysics at the cell surface:  Development of materials to sense
  the forces within us
DTSTART:20120928T101500
DTSTAMP:20260929T050417Z
UID:e07ec955561eb390ab0360a785c2aa45f447e4fd4eaafd09c1294043
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof Khalid Salaita\, Emory University\nThe interplay between 
 physical inputs and biochemical signaling regulates a significant number o
 f biological processes that range from the action of efflux pump transport
 ers to cell adhesion receptors and mounting an immune response.  A major 
 challenge to elucidating chemo-mechanical coupling lies in the development
  of new materials and techniques to measure forces in the cell membrane - 
 which is a essentially a soft interface. In this talk\, I will describe th
 e synthesis and characterization of fluorescence-based turn–on probes fo
 r imaging the molecular tension in living cells in real time ( Stabley et 
 al. Nature Methods 2012). All force gauges require two parts: a spring wit
 h a known force constant\, and an accurate ruler. Likewise\, our sensor ta
 kes advantage of fluorescence resonance energy transfer (FRET) between flu
 orophores as a  molecular "ruler" and flexible polymer chain as a reversi
 ble entropic spring with a known spring constant. The sensor allows one to
  quantify molecular forces (1 –100 pN) with high spatial and temporal re
 solution for a wide range of receptors and cell types using a conventional
  fluorescence microscope. I will describe the application of these sensors
  to image forces associated with clathrin-mediated endocytosis\, Notch rec
 eptor activation\, and integrin adhesion receptors.  Given the superior o
 ptical and physical properties of gold nanoparticles\, we have also develo
 ped second generation gold-nanoparticle-based force sensors and these will
  be described in detail. Finally\, I will discuss the development of the "
 universal force sensor" which employs chemical and enzymatic ligation stra
 tegies to generate recombinantly expressed protein force sensors.
LOCATION:MXF 1 https://plan.epfl.ch/?room==MXF%201
STATUS:CONFIRMED
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