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SUMMARY:Quantitative 3D super-resolution imaging of chromatin ‘blobs’
DTSTART:20210429T133000
DTEND:20210429T143000
DTSTAMP:20260604T000324Z
UID:84db9fdd1157a3c24b898d58cedaaabbc551ad93bdfc3b59aacc7f9b
CATEGORIES:Conferences - Seminars
DESCRIPTION:Lothar Schermelleh\, Ph.D.\, Dept. Biochemistry University of 
 Oxford\nAs affiliated member of the Micron Advanced Bioimaging Unit (www.m
 icronoxford.com)\, he is driving the development of computational analysis
  and fluorescence labelling tools for super-resolution microscopy.\nLothar
 ’s research aims at understanding the relationship between 3D nuclear or
 ganisation and genome activity in mammalian cells by combining genetic too
 ls and advanced optical imaging methods.\nmeeting password:\nhttps://epfl.
 zoom.us/j/88022434967?pwd=OUVHdUY1NERHdlhaMHo4UFc5RTdNUT09\nPasscode: 2413
 83\n\n\nThree-dimensional (3D) chromatin organisation plays a key role in 
 regulating genome function in higher eukaryotes. Despite recognition that 
 the genome partitions into ~1Mb-sized topological associated domains (TADs
 ) based on ensemble Hi-C measurements\, many features of the physical orga
 nisation at the single cell level remain underexplored. In my talk I will 
 present our recent progress in analysing functional chromatin topography a
 nd dynamics on the size scale of TADs using in vivo and in situ 3D sup
 er-resolution microscopy\, supported by 3D scanning electron microscopy. W
 e directly visualise curvilinear arrangements of globular/irregular shaped
  ~200-300 nm diameter nucleosomal condensates with viscoelastic propertie
 s (‘blobs’)\, that are juxtaposed to an RNA-populated chromatin-deplet
 ed interchromatin network. High-content mapping of functional marker reve
 al active/permissive chromatin marker to be confined to a narrow region a
 t blob surfaces\, whereas repressive marks are enriched towards blob int
 eriors. This correlation between nanoscale topology and genome function i
 s temporarily relaxed in postreplicative chromatin\, but remarkably stab
 le after inactivation of cohesin. Our findings establish TAD-sized nanodo
 mains as physical modules of mesoscale genome organisation with function
 al chromatin states being defined by radial position and exposure to a (l
 ikely phase-separated) interchromatin space.
LOCATION:https://epfl.zoom.us/s/88022434967
STATUS:CONFIRMED
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