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SUMMARY:EPFL BioE Talks SERIES  "Nanopipette Biosensors for Single-Cell An
 alysis"
DTSTART:20210329T160000
DTEND:20210329T163000
DTSTAMP:20260506T184540Z
UID:7742dfb415b08c516ff7d3853a8388a823a2b340fac4164be7cb16e4
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Yuri Korchev\, Faculty of Medicine\, Imperial College L
 ondon (UK)\nWEEKLY EPFL BIOE TALKS SERIES\n \n(note that this talk is num
 ber one of a double-feature seminar - see details of the second talk here)
 \n\nAbstract:\nMolecular Biology has advanced we know much about the indiv
 idual molecular components that make up living cells down to the level of 
 the individual atoms. The challenge\, however\, is to fully understand the
  functional integration of these components. This requires determining how
  the molecular machines that make up a living cell are organized and inter
 act together not at the atomic length scale but on a nm scale. To do this 
 we need to develop and applying nanoscale techniques for the visualization
  and quantification of cell machinery in real-time and on living cells. Th
 is will lead to detailed\, quantitative models of sub-cellular structures 
 and molecular complexes under different conditions for both normal and dis
 eased cells.\n\nThis approach ultimately requires the development of novel
  biophysical methods. We have recently pioneered the development of an arr
 ay of new and powerful biophysical tools based on Scanning Ion Conductance
  Microscopy that allow quantitative measurements and non-invasive function
 al imaging of single protein molecules in living cells.\n\nScanning ion co
 nductance microscopy and a battery of associated innovative methods are un
 ique among current imaging techniques\, not only in spatial resolution of 
 living and functioning cells\, but also in the rich combination of imaging
  with other functional and dynamical interrogation methods [1]. There is a
  significant advance to deliver nanotechnological solutions to biosensing 
 that are affordable\, integrated\, fast\, capable of multiplexed detection
  and monitoring\, and crucially to offer high selectivity for the specific
  detection of trace levels of analyte in biological fluids. Herein\, we de
 sign a new class of nanometric field-effect-transistor (FET) sensors [2] a
 nd dubbed nexFET (nanopore extended Field Effect Transistor) [3] that comb
 ine the advantages of nanopore single molecule sensing\, FETs and recognit
 ion chemistry.\n\nThe nexFET biosensors\, with controllable gate voltage e
 nable higher molecular throughput\, enhanced signal-to-noise and even heig
 htened selectivity via functionalization of the nexFET with an embedded re
 ceptor. This is shown for sensitive and selective detection of an anti-ins
 ulin antibody in the presence of its IgG isotype as well as within complex
  mixtures such as blood serum. Self-assembled nanoporous sensors at the ti
 p of nanopipette can be used for simultaneous SICM imaging and chemical im
 aging and can be combined with FET sensors.\n\nReferences:\n[1]. Zhang\, Y
 . et al. (2019). High-resolution label-free 3D mapping of extracellular pH
  of single living cells. Nature Comm. 10\, 1-9\n[2] Y Zhang\, et al. Spear
 head Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis. 
 (2016). ACS Nano 10\, 3214-3221\, DOI: 10.1021/acsnano.5b05211\n[3] R Ren
 \, et al. Nanopore extended field-effect transistor for selective single-m
 olecule biosensing. (2017) Nature Com. 8\, DOI: 10.1038/s41467-017-00549-w
 \n\nBio:\nEducation:\n1983　・B.Sc. in Biology & Physiology\, University
  of St Petersburg\n1984　・M.Sc. in Physiology\, University of St Peters
 burg\n1989　・Ph.D. in Biophysics and Cytology\, Russian Academy of Scie
 nces\nProfessional Career:\n1984 - 1991       Research Scientist\, R
 ussian Academy of Sciences\, St Petersburg\, Russia\n1991 - 1992     
   Wellcome Trust Research Fellow\, St George's Hospital Medical School\, 
 UK\n1992 - 1995       Post-Doctoral Research Fellow\, St George's Ho
 spital Medical School\, London\, UK\n1995 - 1997       Post-Doctoral
  Research Fellow\, Charing Cross & Westminster Medical School\, UK\n1997 -
  2001       Lecturer\, Faculty of Medicine\, Imperial College London
 \, UK\n2001 - 2003       Senior Lecturer\, Faculty of Medicine\, Imp
 erial College London\, UK\n2003 - 2005       Reader\, Faculty of Med
 icine\, Imperial College London\, UK\n2005 - present   Professor\, Facul
 ty of Medicine\, Imperial College London\, UK\n\n\nZoom link (with registr
 ation) for attending remotely: https://go.epfl.ch/EPFLBioETalks\n\n\nIMPOR
 TANT NOTICE: due to restrictions resulting from the ongoing Covid-19 pande
 mic\, this seminar can be followed via Zoom web-streaming only\, (followin
 g prior one-time registration through the link above).
LOCATION:via Zoom web-streaming only\, due to Covid-19 pandemic https://go
 .epfl.ch/EPFLBioETalks https://go.epfl.ch/EPFLBioETalks
STATUS:CONFIRMED
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