BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:EPFL BioE Talks SERIES  "Time Resolved Scanning Ion Conductance Mi
 croscopy: Taking a Gentle Peek at Cell Surface Dynamics"
DTSTART:20210329T163000
DTEND:20210329T170000
DTSTAMP:20260412T084055Z
UID:57bdd3a655e33d86697ac3d34431eba2746f64f6b6c056a3681e7282
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Georg Fantner\, Institute of Biongineering and Institute
  of Microengineering\, EPFL\, Lausanne (CH)\nWEEKLY EPFL BIOE TALKS SERIES
 \n\n(note that this talk is number two of a double-feature seminar - see d
 etails of the first talk here)\n\nAbstract:\nThe evolution of the 3D morph
 ology of cells is at the heart of many biologically relevant process rangi
 ng from stem-cell differentiation\, to cancer metastasis. Things become ev
 en more interesting when looking at the evolution of systems comprising th
 e interaction of multiple cells such as the growth of organoids\, the form
 ation of networks by neurons or the infection of cells by pathogens. When 
 studying these systems\, the information we are after is not just in their
  static structure\, but in how this structure changes over time. Time reso
 lved imaging has proven to be an invaluable tool in this regard. While man
 y excellent optical microscopy techniques exist for time resolved imaging 
 in the sub-micrometer scale at both 2D and 3D\, the options for 3D time la
 pse characterization at the nanometer scale are very limited. Atomic force
  microscopy has long since promised a solution but has failed to deliver e
 xcept for cases of relatively sturdy cells such as bacteria1\,2 or yeast.\
 n\nScanning ion conductance microscopy (SICM) on the other hand has been d
 eveloped specifically for imaging of fragile surfaces of eukaryotic cells3
 . This true non-contact technique is ideally suited for label-free imaging
  of cell surfaces and achieves exquisite resolution down to the nanometer 
 regime4\,5. The challenge to harness this technique for time resolved 3D n
 anocharacterization of living cells lies in the relatively slow imaging sp
 eed of SICM. In this presentation I will show how we apply what we have le
 arned from high-speed AFM to the field of SICM. By reengineering the SICM 
 microscope from the ground up\, we were able to reduce the image acquisiti
 on time for SICM images to 0.5s while extending the imaging duration to da
 ys. I will also discuss the combination of 3D surface data from SICM with 
 2D and 3D volume data from SOFI imaging for correlative high resolution im
 aging of the cell interior as well as the cell membrane6.\n\nReferences:\n
 1. Eskandarian\, H. A. et al. Division site selection linked to inherited 
 cell surface wave troughs in mycobacteria. Nat. Microbiol. 2\, 17094 (2017
 ).\n2. Odermatt\, P. D. et al. Overlapping and essential roles for molecul
 ar and mechanical mechanisms in mycobacterial cell division. Nature Physic
 s (2019) doi:10.1038/s41567-019-0679-1.\n3. Hansma\, P. K.\, Drake\, B.\, 
 Marti\, O.\, Gould\, S. A. & Prater\, C. B. The scanning ion-conductance m
 icroscope. Science (80-. ). 243\, 641–643 (1989).\n4. Novak\, P. et al. 
 Nanoscale live-cell imaging using hopping probe ion conductance microscopy
 . Nat. Methods 6\, 279–281 (2009).\n5. Korchev\, Y. E.\, Bashford\, C. L
 .\, Milovanovic\, M.\, Vodyanoy\, I. & Lab\, M. J. Scanning ion conductanc
 e microscopy of living cells. Biophys. J. 73\, 653–658 (1997).\n6. Navik
 as\, V. et al. Correlative 3D microscopy of single cells using super-resol
 ution and scanning ion-conductance microscopy. bioRxiv (2020) doi:10.1101/
 2020.11.09.374157.\n\n\nBio:\nGEORG E. FANTNER received his MS degree from
  the University of Technology Graz in 2003\, and his PhD degree from UC Sa
 nta Barbara in 2006 (advisor: Paul K. Hansma). During his masters and PhD\
 , he developed a number of high performance AFM instruments and applied th
 em to the study of the molecular origin of bone fracture toughness. After 
 a Postdoc in the biomolecular materials lab at the Massachusetts Institute
  of Technology (Advisor: Angela M. Belcher)\, he joined the École Polytec
 hnique Fédéral de Lausanne as assistant professor in 2010. Now\, as asso
 ciate professor\, he leads the laboratory for bio- and nano-instrumentatio
 n in the institute for bioengineering. His research\, which has been funde
 d by the European Research Council with an ERC starting grant and an ERC c
 onsolidator grant\, focusses on the development of new technologies to mea
 sure and manipulate nanoscale structures in general\, and the development 
 of atomic force microscopy instrumentation in particular. He applies these
  instruments to answer questions in a variety of fields ranging from mater
 ials science and nanotechnology to biology and life science. His interdisc
 iplinary work has been published in many high impact journals such as Natu
 re Materials\, Nature Nanotechnology\, Nature Cell biology\, Nature Microb
 iology\, Nature Communications\, Nano Letters\, and Science\, as well as f
 eatured in a number of popular science- and general-interest magazines. He
  serves as scanning probe microscopy editor for Microscopy and Microanalys
 is (CambridgeCore)\, and as editorial board member for Scientific Reports.
  His recent work focusses on the development of time resolved scanning pro
 be microscopy imaging\, encompassing new modes for high-speed AFM imaging 
 of molecular processes\, as well as long-term time lapse imaging of cellul
 ar processes usomg AFM and scanning ion conductance microscopy. Prof. Fant
 ner hold several patents in the field of nanotechnology and is the co-foun
 der of two nanotechnology companies. Recently he has become active in the 
 field of open hardware\, where he explores new avenues to foster free acad
 emic exchange of knowledge\, particularly for the development of highly so
 phisticated custom instruments.\n\n\nZoom link (with registration) for att
 ending remotely: https://go.epfl.ch/EPFLBioETalks\n\n\nIMPORTANT NOTICE: d
 ue to restrictions resulting from the ongoing Covid-19 pandemic\, this sem
 inar can be followed via Zoom web-streaming only\, (following prior one-ti
 me registration through the link above).
LOCATION:via Zoom web-streaming only\, due to Covid-19 pandemic https://go
 .epfl.ch/EPFLBioETalks https://go.epfl.ch/EPFLBioETalks
STATUS:CONFIRMED
END:VEVENT
END:VCALENDAR