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SUMMARY:Harald HESS: 3D Imaging of Cells by FIBSEM with Correlation to Cry
 o Fluorescence Microscopy
DTSTART:20210624T170000
DTEND:20210624T180000
DTSTAMP:20260916T044106Z
UID:3864b49d2df52e70af39b783e28664e4cd6cee884ae3d39d4225b3f5
CATEGORIES:Conferences - Seminars
DESCRIPTION:Harald Hess\, HHMI’s Janelia Research Campus\, USA \nThis e
 vent is part of the EPFL Seminar Series in Imaging (https://imagingseminar
 s.org).\n\nAbstract. 3D electron microscopy data can be acquired by Focuse
 d Ion Beam Scanning Electron Microscopy (FIB-SEM) where fine sequences of 
 4-8 nm increments are ablated off of a sample surface and each such surfac
 e is imaged with the SEM. At the finest resolution and with month-long sta
 ble operation\, comprehensive whole cells can be acquired that transcend t
 he limited cut section views of traditional TEM used in biology.\n\nSevera
 l examples of such data are presented along with the potential that segmen
 tation offers to explore and formulate biological questions. Correlative m
 icroscopy can be achieved by a cryogenic protocol where samples are vitrif
 ied\, imaged with PALM or SIM at low temperatures followed by EM staining 
 and FIBSEM.  A 3D registration procedure can keep most position errors be
 tween PALM and EM data at ~ 30 nm.  Examples validating the approach with
  mitochondrial and endoplasmic reticulum labels are presented along with e
 xamples showcasing how unknown vesicle types and other structures can be i
 dentified by an associated protein.\n\nBiography. Harald Hess is a senior 
 group leader at HHMI’s Janelia Research Campus in the USA. His main int
 erests lie in the development of high-throughput 3D electron microscopy an
 d super-resolution 3D optical microscopy for use in brain connectomics and
  cell biology.  
LOCATION:https://epfl.zoom.us/j/83704327175
STATUS:CONFIRMED
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