BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:EPFL BioE Talks SERIES  "Streamlining Fragment-Based Chemoproteomi
 cs for in Cell Screening of Large Electrophile Libraries"
DTSTART:20210510T160000
DTEND:20210510T163000
DTSTAMP:20260408T115634Z
UID:9b06342ec28138fb2610b52434e440f9fa5c2a128233f6433ed03bb5
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Steven Gygi\, Department of Cell Biology\, Harvard Medi
 cal School\, Boston\, MA (USA)\nWEEKLY EPFL BIOE TALKS SERIES\n \n(note t
 hat this talk is number one of a double-feature seminar - see details of t
 he second talk here)\n\nAbstract:\nUnderstanding the cellular role of each
  human protein requires the development of small-molecule reagents that mo
 dulate protein interactions\, expression\, and function. Current methods\,
  however\, lack the throughput needed to screen large chemical libraries f
 or interactions across the entire proteome. To facilitate this endeavor\,
  we have redesigned the entire workflow for activity-based protein profili
 ng (ABPP) of reactive cysteine residues with an eye towards screening larg
 e libraries. We broadly screened cysteine reactivity for a library of 285 
 electrophiles in duplicate using intact HCT116 cells in just 4 days. More 
 than 20\,000 cysteines were quantified with >6\,000 proteins harboring rea
 ctive cysteine sites. We have repeated the screen in 3 additional lines an
 d have started to screen a full library of 3\,200 compounds in K562 cells.
 \n\nBio:\nSteven Gygi received his Ph.D. in Pharmacology and Toxicology 
 from the University of Utah in 1995.  He performed postdoctoral work with
  Ruedi Aebersold at the University of Washington from 1996-2000 studying m
 ass spectrometry-based proteomics.  Today\, his lab at Harvard Medical Sc
 hool develops proteomic technologies to systematically measure protein pro
 perties including their expression levels\, interactions\, and modificatio
 ns.  Sample multiplexing using tandem mass tags (TMT) currently allows 18
  different cellular states to be simultaneously analyzed in his lab.\n\n\n
 Zoom link (with registration) for attending remotely: https://go.epfl.ch/E
 PFLBioETalks\n\n\nIMPORTANT NOTICE: due to restrictions resulting from the
  ongoing Covid-19 pandemic\, this seminar can be followed via Zoom web-str
 eaming only\, (following prior one-time registration through the link abov
 e).
LOCATION:via Zoom web-streaming only\, due to Covid-19 pandemic https://go
 .epfl.ch/EPFLBioETalks https://go.epfl.ch/EPFLBioETalks
STATUS:CONFIRMED
END:VEVENT
END:VCALENDAR