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SUMMARY:Impaired Differentiation: Understanding a Single Cell State Transi
 tion
DTSTART:20210705T100000
DTSTAMP:20260407T025944Z
UID:3ffdd7d2078ca222ad2ec68d816f76764f86f96e5754b5f05fa72718
CATEGORIES:Conferences - Seminars
DESCRIPTION:Merrit Romeike from Christa Buecker lab at MaxPerutzLabs Vienn
 a\nDevelopment is characterized by distinct\, coordinated cell state trans
 itions. During each transition\, an existing gene expression program is di
 smantled\, and a new cellular identity has to be established.\n\nWe use th
 e exit from naïve pluripotency as a highly accessible and controllable mo
 del system for a single cell state transition: mouse embryonic stem cells 
 cultivated under defined conditions are homogenous naïve pluripotent and 
 maintained by a well-established core gene regulatory network. Upon change
  of culture conditions\, this network is rapidly dismantled\, and cells ir
 reversibly commit to differentiation into formative pluripotency\, a less 
 characterized cell state. Despite extensive screening for factors required
  for exiting naïve pluripotency\, so far not a single factor has been ide
 ntified which completely abrogates differentiation ability. Differentiatio
 n impaired mutants rather exhibit a phenotype which is mostly described as
  prolonged expression of pluripotency markers or clustering of bulk transc
 ription profiles with naïve wildtype.\n\nSingle cell methods open up a wi
 ndow into understanding these aberrant cell states. We collected fine-tune
 d differentiation time courses of control and selected differentiation imp
 aired mutants\, targeting the major signalling pathways involved in this t
 ransition. We built a shared trajectory across these genotypes and could r
 ecapitulate a delay in cell state of mutants in comparison to time matched
  control cells. With this\, we now address fundamental questions: are obse
 rved differences based on differences in cell state\, or are they directly
  affected by the altered gene regulatory networks? How are these networks 
 responding to the challenge of genetic manipulations? How is the remarkabl
 e robustness of this cell state transition ensured?\n\nTaken together\, ou
 r work will elucidate the mechanism of a single cell state transition and 
 its intrinsic robustness.\n 
LOCATION:https://epfl.zoom.us/j/65615080775?pwd=R1A5cEQwZU81UUNGMndQNXBLWC
 tKdz09
STATUS:CONFIRMED
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