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SUMMARY:ChemBio e-seminar by Prof. Sarah Slavoff (Yale University): Dark M
 atter of the Human Genome
DTSTART:20210928T161500
DTEND:20210928T171500
DTSTAMP:20260405T205239Z
UID:70905bc22a0b3ef8df5a2a61aec2c8245a63fda568cf07219e9ab93d
CATEGORIES:Conferences - Seminars
DESCRIPTION:https://chem.yale.edu/people/sarah-slavoff\nTitle: Dark Matter
  of the Human Genome\n\nAbstract: Advanced methods in next-generation sequ
 encing and proteogenomics have revealed thousands of previously invisible 
 human genes\, increasing the known size of the human genome by at least 10
 %. This previously unannotated “dark matter” of the human genome inclu
 des small open reading frames (smORFs) encoding polypeptides of fewer than
  100 amino acids\, and alternative open reading frames (alt-ORFs) encoding
  proteins 100 amino acids or larger. Sm/alt-ORFs previously escaped detect
 ion due to their short lengths\, overlap with annotated protein coding seq
 uences in different reading frames\, and/or initiation with non-AUG start 
 codons. Recent studies have shown that hundreds of smORFs are required for
  cell growth and survival\, and some smORF-encoded polypeptides (SEPs) bin
 d to and regulate the activity of macromolecular complexes involved in cri
 tical cellular processes and disease. However\, key questions about SEP mo
 difications\, detection\, and functions remain. My research group is devel
 oping advanced mass spectrometry proteomics-based approaches to answer eac
 h of these questions\, and in this presentation\, I will describe (1) prot
 eomic discovery of alt-RPL36\, which overlaps the coding sequence for huma
 n ribosomal protein L36 and binds to TMEM24 to regulate PI3K/AKT/mTOR path
 way signaling\, and (2) identification of phosphorylation sites on the NBD
 Y SEP that control membraneless organelle dynamics. Taken together\, these
  findings demonstrate that interfacing phosphoproteomics\, interactomics a
 nd chemoproteomics with proteogenomics can drive forward our understanding
  of the cellular functions of recently discovered sm/alt-ORF-encoded polyp
 eptides and proteins. \n\nSpeaker's Biography: Sarah Slavoff received her
  Bachelor's degree in Biochemistry from the University of Maryland\, Colle
 ge Park\, followed by Ph.D. studies at MIT with Professor Alice Ting durin
 g which she developed new technologies for enzyme-mediated protein bioconj
 ugation. Sarah's postdoctoral fellowship with Alan Saghatelian at Harvard 
 University resulted in the first proteomic technology for large-scale dete
 ction of previously invisible polypeptides expressed in human cells\, whic
 h are now known to number at least in the thousands. Sarah started her ind
 ependent research group at Yale in 2014\, and was promoted to Associate Pr
 ofessor in 2021. Her research program focuses on advanced (chemo)proteomic
  technologies to detect and characterize human polypeptide-encoding genes.
  She has been recognized with a Searle Scholar Award and a Yale Arthur Gre
 er Memorial Prize for Scholarly Research.\n\nLab website: https://slavoffl
 ab.yale.edu/publications-yale
LOCATION:https://epfl.zoom.us/j/63707358272
STATUS:CONFIRMED
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