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SUMMARY:BMI Seminar // Hidenobu Mizuno - Imaging neuronal morphology and a
 ctivity pattern in developing cerebral cortex layer 4
DTSTART:20211027T121500
DTEND:20211027T131500
DTSTAMP:20260506T044848Z
UID:de2f19149c50832dcd5d4734953fe15b1418d46a8e00ba573d0708b0
CATEGORIES:Conferences - Seminars
DESCRIPTION:Hidenobu Mizuno\, Kumamoto University\, Japan\nIMPORTANT NOTIC
 E:\n\nIn-person attendance of this seminar is subjected to some constraint
 s:\n\n	Maximum number of participants is limited to 80 \n	Valid COVID cert
 ificate and ID (e.g. Camipro card)\, required to enter the conference room
 \, will be checked at the entrance\n	Face masks are mandatory for everyone
  in the seminar room (excepted the speaker while presenting).\n\nEstablish
 ment of precise neuronal connectivity in the neocortex relies on activity-
 dependent circuit reorganization during postnatal development. In the mous
 e somatosensory cortex layer 4\, barrels are arranged in one-to-one corres
 pondence to whiskers on the face. Thalamocortical axon termini are cluster
 ed in the center of each barrel. The layer 4 spiny stellate neurons are lo
 cated around the barrel edge\, extend their dendrites primarily toward the
  barrel center\, and make synapses with thalamocortical axons correspondin
 g to a single whisker. These organized circuits are established during the
  first postnatal week through activity-dependent refinement processes. How
 ever\, activity pattern regulating the circuit formation is still elusive.
  Using two-photon calcium imaging in living neonatal mice\, we found that 
 layer 4 neurons within the same barrel fire synchronously in the absence o
 f peripheral stimulation\, creating a ''patchwork'' pattern of spontaneous
  activity corresponding to the barrel map. We also found that disruption o
 f GluN1\, an obligatory subunit of the N-methyl-D-aspartate (NMDA) recepto
 r\, in a sparse population of layer 4 neurons reduced activity correlation
  between GluN1 knockout neuron pairs within a barrel. Our results provide 
 evidence for the involvement of layer 4 neuron NMDA receptors in spatial o
 rganization of the spontaneous firing activity of layer 4 neurons in the n
 eonatal barrel cortex. In the talk I will introduce our strategy to analyz
 e the role of NMDA receptor-dependent correlated activity in the layer 4 c
 ircuit formation.\n 
LOCATION:SV 1717 (masks & COVID pass requested) https://plan.epfl.ch/?room
 ==SV%201717 https://epfl.zoom.us/j/96263948305
STATUS:CONFIRMED
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