Event List
retrieve:
Return the details about the given Event id.
list:
List all Event objects.
GET /api/v1/events/?format=api&offset=30&ordering=-event__start_time
{ "count": 238, "next": "https://memento.epfl.ch/api/v1/events/?format=api&limit=10&offset=40&ordering=-event__start_time", "previous": "https://memento.epfl.ch/api/v1/events/?format=api&limit=10&offset=20&ordering=-event__start_time", "results": [ { "id": 71476, "title": "Plasmonic Nanostructure Biosensors for Continuous Monitoring and Multiplexed Profiling with Ultra-Broad Dynamic Range", "slug": "plasmonic-nanostructure-biosensors-for-continuou-2", "event_url": "https://memento.epfl.ch/event/plasmonic-nanostructure-biosensors-for-continuou-2", "visual_url": "https://memento.epfl.ch/image/32821/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32821/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32821/max-size.jpg", "lang": "en", "start_date": "2026-05-01", "end_date": "2026-05-01", "start_time": "17:00:00", "end_time": null, "description": "<p>Thesis Director: Prof. H. Altug,<br>\r\nBiotechnology and Bioengineering doctoral program<br>\r\nThesis Nr. 11156<br>\r\n<br>\r\nTo take part in the public defense, please contact directly the speaker</p>", "image_description": "", "creation_date": "2026-03-27T12:14:00", "last_modification_date": "2026-03-31T13:42:48", "link_label": "", "link_url": "", "canceled": "True", "cancel_reason": "en même temps que Balelec", "place_and_room": "MXF 1", "url_place_and_room": "https://plan.epfl.ch/?room==MXF%201", "url_online_room": "", "spoken_languages": [], "speaker": "<strong>Abtin SAATEH</strong>", "organizer": "", "contact": "<strong>Abtin SAATEH</strong>", "is_internal": "False", "theme": "", "vulgarization": { "id": 1, "fr_label": "Tout public", "en_label": "General public" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "EDBB", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/120214/", "category": { "id": 12, "code": "SOUTE", "fr_label": "Soutenances de thèses", "en_label": "Thesis defenses", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/9/?format=api" ] }, { "id": 71495, "title": "Reprogramming GPCR Specificity: Engineering CXCR2 for Selective CXCL5 Binding and Cellular Signaling", "slug": "reprogramming-gpcr-specificity-engineering-cxcr2-2", "event_url": "https://memento.epfl.ch/event/reprogramming-gpcr-specificity-engineering-cxcr2-2", "visual_url": "https://memento.epfl.ch/image/32837/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32837/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32837/max-size.jpg", "lang": "en", "start_date": "2026-04-30", "end_date": "2026-04-30", "start_time": "17:00:00", "end_time": null, "description": "<p>Thesis Director: Prof. P. D. Barth,<br>\r\nBiotechnology and Bioengineering doctoral program<br>\r\nThesis Nr. 11393<br>\r\n<br>\r\nTo take part in the public defense, please contact directly the speaker</p>", "image_description": "", "creation_date": "2026-03-30T16:59:07", "last_modification_date": "2026-03-30T16:59:08", "link_label": "", "link_url": "", "canceled": "False", "cancel_reason": "", "place_and_room": "CM 1 2", "url_place_and_room": "https://plan.epfl.ch/?room==CM%201%202", "url_online_room": "", "spoken_languages": [], "speaker": "<strong>Ana Paola RICO VILLARREAL</strong>", "organizer": "", "contact": "<strong>Ana Paola RICO VILLARREAL</strong>", "is_internal": "False", "theme": "", "vulgarization": { "id": 1, "fr_label": "Tout public", "en_label": "General public" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "EDBB", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/120237/", "category": { "id": 12, "code": "SOUTE", "fr_label": "Soutenances de thèses", "en_label": "Thesis defenses", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/9/?format=api" ] }, { "id": 71516, "title": "Xenon Dynamic Nuclear Polarization: from Spin Dynamics to In-Vivo Applications", "slug": "xenon-dynamic-nuclear-polarization-from-spin-dyn-2", "event_url": "https://memento.epfl.ch/event/xenon-dynamic-nuclear-polarization-from-spin-dyn-2", "visual_url": "https://memento.epfl.ch/image/32852/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32852/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32852/max-size.jpg", "lang": "en", "start_date": "2026-04-24", "end_date": "2026-04-24", "start_time": "17:00:00", "end_time": null, "description": "<p>Thesis Directors: Prof. R. Gruetter, Dr A. Capozzi<br>\r\nPhysics doctoral program<br>\r\nThesis Nr. 11427<br>\r\n<br>\r\nTo take part in the public defense, please contact directly the speaker</p>", "image_description": "", "creation_date": "2026-04-01T14:42:56", "last_modification_date": "2026-04-01T14:42:58", "link_label": "", "link_url": "", "canceled": "False", "cancel_reason": "", "place_and_room": "CE 1 4", "url_place_and_room": "https://plan.epfl.ch/?room==CE%201%204", "url_online_room": "https://epfl.zoom.us/j/66418358152?pwd=kRVVP5y6NGjH4pDROhIPv7t0zIpSvI.1", "spoken_languages": [], "speaker": "<strong>Emma Linnea WISTRÖM</strong>", "organizer": "", "contact": "<strong>Emma Linnea WISTRÖM</strong>", "is_internal": "False", "theme": "", "vulgarization": { "id": 1, "fr_label": "Tout public", "en_label": "General public" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "EDPY", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/120266/", "category": { "id": 12, "code": "SOUTE", "fr_label": "Soutenances de thèses", "en_label": "Thesis defenses", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/5/?format=api" ] }, { "id": 71514, "title": "Plasmonic Nanostructure Biosensors for Continuous Monitoring and Multiplexed Profiling with Ultra-Broad Dynamic Range", "slug": "plasmonic-nanostructure-biosensors-for-continuou-4", "event_url": "https://memento.epfl.ch/event/plasmonic-nanostructure-biosensors-for-continuou-4", "visual_url": "https://memento.epfl.ch/image/32850/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32850/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32850/max-size.jpg", "lang": "en", "start_date": "2026-04-27", "end_date": "2026-04-27", "start_time": "17:00:00", "end_time": null, "description": "<p>Thesis Director: Prof. H. Altug,<br>\r\nBiotechnology and Bioengineering doctoral program<br>\r\nThesis Nr. 11156<br>\r\n<br>\r\nTo take part in the public defense, please contact directly the speaker</p>", "image_description": "", "creation_date": "2026-04-01T14:17:26", "last_modification_date": "2026-04-01T14:17:27", "link_label": "", "link_url": "", "canceled": "False", "cancel_reason": "", "place_and_room": "MXF 1", "url_place_and_room": "https://plan.epfl.ch/?room==MXF%201", "url_online_room": "", "spoken_languages": [], "speaker": "<strong>Abtin SAATEH</strong>", "organizer": "", "contact": "<strong>Abtin SAATEH</strong>", "is_internal": "False", "theme": "", "vulgarization": { "id": 1, "fr_label": "Tout public", "en_label": "General public" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "EDBB", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/120262/", "category": { "id": 12, "code": "SOUTE", "fr_label": "Soutenances de thèses", "en_label": "Thesis defenses", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/9/?format=api" ] }, { "id": 71515, "title": "Application of a multi-rate state observer for real-time plasma density reconstruction and control on TCV", "slug": "application-of-a-multi-rate-state-observer-for-r-2", "event_url": "https://memento.epfl.ch/event/application-of-a-multi-rate-state-observer-for-r-2", "visual_url": "https://memento.epfl.ch/image/32851/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32851/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32851/max-size.jpg", "lang": "en", "start_date": "2026-04-10", "end_date": "2026-04-10", "start_time": "17:00:00", "end_time": null, "description": "<p>Thesis Directors: Dr. O. Sauter, Dr F. A. A. Felici<br>\r\nPhysics doctoral program<br>\r\nThesis Nr. 11288<br>\r\n<br>\r\nTo take part in the public defense, please contact directly the speaker</p>", "image_description": "", "creation_date": "2026-04-01T14:32:11", "last_modification_date": "2026-04-01T14:32:56", "link_label": "", "link_url": "", "canceled": "False", "cancel_reason": "", "place_and_room": "CE 1 3", "url_place_and_room": "https://plan.epfl.ch/?room==CE%201%203", "url_online_room": "", "spoken_languages": [], "speaker": "<strong>Francesco PASTORE</strong>", "organizer": "", "contact": "<strong>Francesco PASTORE</strong>", "is_internal": "False", "theme": "", "vulgarization": { "id": 1, "fr_label": "Tout public", "en_label": "General public" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "EDPY", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/120264/", "category": { "id": 12, "code": "SOUTE", "fr_label": "Soutenances de thèses", "en_label": "Thesis defenses", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/5/?format=api" ] }, { "id": 71172, "title": "Anisotropic Inviscid Limit for the Navier-Stokes Equations with Transport Noise Between Two Plates", "slug": "anisotropic-inviscid-limit-for-the-navier-stokes-e", "event_url": "https://memento.epfl.ch/event/anisotropic-inviscid-limit-for-the-navier-stokes-e", "visual_url": "https://memento.epfl.ch/image/32540/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32540/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32540/max-size.jpg", "lang": "en", "start_date": "2026-03-24", "end_date": "2026-05-25", "start_time": "16:30:00", "end_time": "17:30:00", "description": "<p>We investigate an anisotropic vanishing viscosity limit of the 3D stochastic Navier-Stokes equations posed between two horizontal plates, with Dirichlet no-slip boundary condition. The turbulent viscosity is split into horizontal and vertical directions, each of which approaches zero at a different rate. The underlying Cylindrical Brownian Motion driving our transport-stretching noise is decomposed into horizontal and vertical components, which are scaled by the square root of the respective directional viscosities. We prove that if the ratio of the vertical to horizontal viscosities approaches zero, then there exists a sequence of weak martingale solutions convergent to the strong solution of the deterministic Euler equation on its lifetime of existence. A particular challenge is that the anisotropic scaling ruins the divergence-free property for the spatial correlation functions of the noise.</p>", "image_description": "", "creation_date": "2026-02-18T12:59:24", "last_modification_date": "2026-03-22T19:58:56", "link_label": "Working-Reading Seminar", "link_url": "https://www.epfl.ch/labs/stoan/reading-working-seminar/", "canceled": "True", "cancel_reason": "", "place_and_room": "MA B2 485", "url_place_and_room": "", "url_online_room": "", "spoken_languages": [ "https://memento.epfl.ch/api/v1/spoken_languages/2/?format=api" ], "speaker": "Dr Daniel Goodair (EPFL)", "organizer": "<a href=\"https://people.epfl.ch/xue-mei.li\">Xue-Mei Li</a>", "contact": "<a href=\"https://people.epfl.ch/xue-mei.li\">Xue-Mei Li</a>", "is_internal": "False", "theme": "", "vulgarization": { "id": 3, "fr_label": "Expert", "en_label": "Expert" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "Working-Reading seminar, Probability, stochastic analysis", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/119781/", "category": { "id": 1, "code": "CONF", "fr_label": "Conférences - Séminaires", "en_label": "Conferences - Seminars", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/7/?format=api" ] }, { "id": 70936, "title": "Probing and Modulating Transcription Factor–DNA Interactions via Chemically Modified Proteins", "slug": "probing-and-modulating-transcription-factordna-i-2", "event_url": "https://memento.epfl.ch/event/probing-and-modulating-transcription-factordna-i-2", "visual_url": "https://memento.epfl.ch/image/32324/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32324/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32324/max-size.jpg", "lang": "en", "start_date": "2026-05-19", "end_date": "2026-05-19", "start_time": "16:15:00", "end_time": "17:45:00", "description": "<p>Chemical protein synthesis provides a powerful means to prepare novel, modified proteins with atomic-level precision, offering unprecedented opportunities to understand fundamental biological processes.<sup>1</sup> Of particular interest is gene expression, which is controlled through interactions between transcription factors (TFs) and DNA. This presentation will highlight the power of combining total synthesis and late-stage transformations to generate complex, modified proteins for deciphering the molecular roles of post-translational modifications (PTMs) in TFs regulation. Specific examples will focus on the synthesis of site-specifically phosphorylated and acetylated TFs, such as the Myc/Max system.<sup>2, 3</sup> Remarkably, these studies revealed that phosphorylation and acetylation patterns modulate Max–DNA interactions by altering DNA binding affinity and sequence specificity. Importantly, such mechanistic insights led to the development of novel bioactive miniproteins derived from Max (μMax), capable of inhibiting oncogene expression and cancer cell proliferation through antagonistic binding to target genes in cancer cells, paving the way for the development of new therapeutic proteins targeting oncogene expression.<sup>4-6</sup><br>\r\n<br>\r\n<br>\r\n1. O. Harel, M. Jbara, <em>Angew. Chem. Int. Ed., </em><strong>2023</strong>, <em>62</em>, e202217716<br>\r\n2. R. Nithun, Y. Yao, X. Lin, S. Habiballah, A. Afek, M. Jbara, <em>Angew. Chem. In. Ed., </em><strong>2023</strong>,<em> 62,</em> e202310913<br>\r\n3. R. Nithun, Y. Yao, O. Harel, S. Habiballah, A. Afek, M. Jbara, <em>ACS Central Science, </em><strong>2024</strong>, <em>10</em>, 1295–1303<br>\r\n4. X. Lin, S. Mandal, R. Nithun, R. Kolla, B. Bouri, H. Lashuel, M. Jbara, <em>JACS</em>, <strong>2024</strong>, 146, 25788<br>\r\n5. X. Lin, O. Harel, M. Jbara, <em>Angew. Chem. In. Ed.,</em> <strong>2024</strong>, <em>63,</em> e202317511<br>\r\n6. O. Harel, F. Nadal-Bufi, R. Nithun, Y. Yao, A. Afek, M. Vendrell, M. Jbara, <em>JACS</em>, <strong>2025</strong>, 147, 46, 42647</p>", "image_description": "", "creation_date": "2026-01-23T10:10:50", "last_modification_date": "2026-03-10T12:19:13", "link_label": "", "link_url": "", "canceled": "False", "cancel_reason": "", "place_and_room": "BCH 2218", "url_place_and_room": "https://plan.epfl.ch/?room==BCH%202218", "url_online_room": "", "spoken_languages": [ "https://memento.epfl.ch/api/v1/spoken_languages/2/?format=api" ], "speaker": "<a href=\"https://www.jbaralab.sites.tau.ac.il/mj\">Dr Muhammad Jbara</a> (Tel Aviv University)", "organizer": "Professor Fierz Beat", "contact": "Marie Munoz", "is_internal": "False", "theme": "", "vulgarization": { "id": 2, "fr_label": "Public averti", "en_label": "Informed public" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "CBseminar", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/119416/", "category": { "id": 1, "code": "CONF", "fr_label": "Conférences - Séminaires", "en_label": "Conferences - Seminars", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/14/?format=api" ] }, { "id": 71443, "title": "Mathematics Colloquium", "slug": "mathematics-colloquium-13", "event_url": "https://memento.epfl.ch/event/mathematics-colloquium-13", "visual_url": "https://memento.epfl.ch/image/32790/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32790/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32790/max-size.jpg", "lang": "en", "start_date": "2026-06-17", "end_date": "2026-06-17", "start_time": "16:15:00", "end_time": "17:15:00", "description": "<strong>Title</strong>: <br>\r\nPositive random walks and positive-semidefinite random matrices<br>\r\n<br>\r\n<strong>Abstract</strong>:<br>\r\nOn the real line, a random walk that can only move in the positive direction is very unlikely to remain close to its origin. After a fixed number of steps, the left tail has a Gaussian profile under minimal assumptions. Remarkably, a similar phenomenon occurs when we consider a positive random walk on the cone of positive-semidefinite matrices. After a fixed number of steps, the minimum eigenvalue is described by a Gaussian random matrix model.<br>\r\nThis talk introduces a new way to make this intuition rigorous. The methodology addresses an open problem in computational mathematics about sparse random embeddings. The presentation targets a general mathematical audience.<br>\r\nPreprint: <a href=\"https://arxiv.org/abs/2501.16578\" target=\"_blank\">https://arxiv.org/abs/2501.16578</a><br>\r\n<br>\r\n<strong>Please register on the following form : </strong>https://forms.gle/ppXwKo9HmTgFk3rc7", "image_description": "", "creation_date": "2026-04-01T11:03:30", "last_modification_date": "2026-04-01T11:03:45", "link_label": "", "link_url": "", "canceled": "False", "cancel_reason": "", "place_and_room": "BCH 2201", "url_place_and_room": "https://plan.epfl.ch/?room==BCH%202201", "url_online_room": "", "spoken_languages": [ "https://memento.epfl.ch/api/v1/spoken_languages/2/?format=api" ], "speaker": "Prof. Joel A. Tropp, Steele Family Professor of Applied & Computational Mathematics, Caltech", "organizer": "Prof. Nicolas Boumal", "contact": "Institute of Mathematics", "is_internal": "True", "theme": "", "vulgarization": { "id": 2, "fr_label": "Public averti", "en_label": "Informed public" }, "registration": { "id": 2, "fr_label": "Sur invitation", "en_label": "Invitation required" }, "keywords": "", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/120257/", "category": { "id": 1, "code": "CONF", "fr_label": "Conférences - Séminaires", "en_label": "Conferences - Seminars", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/402/?format=api", "https://memento.epfl.ch/api/v1/mementos/5/?format=api", "https://memento.epfl.ch/api/v1/mementos/7/?format=api" ] }, { "id": 71500, "title": "IMX Talks - Sustainable green bio-polycarbonates from bio-based feedstocks and CO2 toward carbon-neutral goals", "slug": "imx-talks-sustainable-green-bio-polycarbonates-f-2", "event_url": "https://memento.epfl.ch/event/imx-talks-sustainable-green-bio-polycarbonates-f-2", "visual_url": "https://memento.epfl.ch/image/32842/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/32842/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/32842/max-size.jpg", "lang": "en", "start_date": "2026-04-16", "end_date": "2026-04-16", "start_time": "16:15:00", "end_time": "17:00:00", "description": "<p>The growing challenges of plastic pollution, fossil resource depletion, and carbon emissions have accelerated the search for sustainable alternatives to polymers. This presentation will focus on the development of fully bio-based polycarbonates, synthesized through the polymerization of carbon dioxide with renewable epoxides. We will first detail our laboratory’s work in generating a diverse portfolio monomer from a wide range of bio-feedstocks including plant oils, terpenes, lignocellulosic biomass, and industrial byproducts. Another focus is the effect of monomer structure on resulting polymer properties and performance, and how post-polymerization modification strategies can be employed to tailor materials for specific applications. (2) Applications of bio-based polycarbonates will be then discussed in fields such as coatings, biomedical materials, adhesives, and their recycling pathways. (3) An outline of future directions will be explored, emphasizing the roles of advanced catalyst design, AI-driven manufacturing, and supportive policy frameworks to enable scalable and sustainable production. The integration of CO<sub>2</sub> utilization with renewable feedstocks presents a compelling route toward circular and carbon–neutral materials.<br>\r\n<br>\r\nBio: Shaoqing Cui, Ph.D., is a Principal Investigator under the Zhejiang University’s \"Hundred Talents Program\" and a recipient of China’s National High-level Talent Program - (Overseas Young Talent Project). She used to be an Assistant Professor at the University of Tennessee-Knoxville (United States) before joining Zhejiang University. Her research focuses on the valorization of agricultural waste and carbon dioxide conversion and utilization, including high-value utilization of agricultural straw, waste oils as well as microalgae biomass. She has led and participated in multiple research projects, including a U.S. Department of Energy project on the efficient catalytic conversion of waste-treatment microalgae and CO<sub>2</sub> into high-value plastic products, also two U.S. Department of Agriculture projects. Since joining the Zhejiang University in 2023, she has also been leading several major research initiatives, including projects funded by the National Natural Science Foundation of China and Zhejiang Provincial Key R&D Programs.</p>", "image_description": "", "creation_date": "2026-03-31T08:57:25", "last_modification_date": "2026-03-31T08:57:39", "link_label": "Website", "link_url": "https://person.zju.edu.cn/en/0022234", "canceled": "False", "cancel_reason": "", "place_and_room": "MXF 312", "url_place_and_room": "https://plan.epfl.ch/?room==MXF%20312", "url_online_room": "", "spoken_languages": [ "https://memento.epfl.ch/api/v1/spoken_languages/2/?format=api" ], "speaker": "Dr. Shaoqing Cui, Zhejiang University, China", "organizer": "Prof. Tiffany Abitbol", "contact": "Prof. Tiffany Abitbol", "is_internal": "False", "theme": "", "vulgarization": { "id": 1, "fr_label": "Tout public", "en_label": "General public" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/120245/", "category": { "id": 1, "code": "CONF", "fr_label": "Conférences - Séminaires", "en_label": "Conferences - Seminars", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/8/?format=api", "https://memento.epfl.ch/api/v1/mementos/314/?format=api" ] }, { "id": 70233, "title": "Cryo electron microscopy of vitreous sections revisited for high throughput tomographic imaging of tissue and cells", "slug": "cryo-electron-microscopy-of-vitreous-sections-revi", "event_url": "https://memento.epfl.ch/event/cryo-electron-microscopy-of-vitreous-sections-revi", "visual_url": "https://memento.epfl.ch/image/31688/200x112.jpg", "visual_large_url": "https://memento.epfl.ch/image/31688/720x405.jpg", "visual_maxsize_url": "https://memento.epfl.ch/image/31688/max-size.jpg", "lang": "en", "start_date": "2026-05-05", "end_date": "2026-05-05", "start_time": "16:00:00", "end_time": "17:00:00", "description": "<br>\r\n<br>\r\nCellular cryo electron tomography is experiencing spectacular developments, revealing with increasing resolution and complexity the structures and interactions of macromolecules at work in living matter. To obtain thin samples from biological tissue and cells in their flash-frozen native state, cryo-FIB milling is nowadays the most popular method, with impressive successes. The alternative, cryo-ultramicrotomy - also known as CEMOVIS - has been overshadowed because of (i) technical difficulties associated to cryo-section generation and collection - in particular the lack of control over adhesion; and (ii) crevasses and compression artefacts.<br>\r\nI will present recent developments with optimization of the experimental workflow, focusing on section adhesion and their support grids. These result in high throughput data acquisition, with typically several tens to hundreds of cryo-tomograms per sample. Revisiting cutting artefacts shows that crevasses alter but 10 % of the sample volume. Compression is heterogeneous, with scale and material-dependent effects. This is altogether a drawback – with no simple computational correction – and an advantage for projects dedicated to non-(or little)-affected objects such as nucleosomes and chromatin.<br>\r\nAltogether, it is time to revive CEMOVIS as a practical alternative to cryo-FIB milling, offering specific advantages: (i) imaging of large areas of any type of sample, from cells to tissues, organs or even entire organisms – and more generally all kinds of hydrated molecular systems ; (ii) the possibility of serial section approaches ; (iii) no beam damage ; and (iv) a specimen thickness that can be tuned down to 30-50 nm, which may be an advantage for high resolution imaging of small molecular complexes.<br>\r\n<br>\r\n<br>\r\n<br>\r\n<br>\r\n<strong>Biography</strong><br>\r\n<br>\r\nAmélie Leforestier is a CNRS research director at the Solid-State Physics Laboratory (LPS, Paris-Saclay, France). After a PhD in Life Sciences on liquid crystalline self-assembly of DNA, she learned cryo electron microscopy during her post-doctoral studies in the group of Jacques Dubochet in Lausanne. Back in France, she set up a cryo-EM facility for soft matter physics at LPS. She is part of an <a href=\"https://equipes.lps.u-psud.fr/sobio/\">interdisciplinary team</a> dedicated to self-assembly processes at work in living systems at the molecular level. Her work, combining concepts from soft matter physics (poly-electrolytes, liquid crystals, frustration) and structural biology, focuses on the geometry and interactions in condensed states of DNA and chromosomes <em>in vitro</em> and <em>in situ</em>.", "image_description": "", "creation_date": "2025-10-29T08:00:54", "last_modification_date": "2026-03-04T11:07:41", "link_label": "", "link_url": "", "canceled": "False", "cancel_reason": "", "place_and_room": "", "url_place_and_room": "", "url_online_room": "https://epfl.zoom.us/j/65708791562", "spoken_languages": [ "https://memento.epfl.ch/api/v1/spoken_languages/2/?format=api" ], "speaker": "Amélie Leforestier | CNRS, France", "organizer": "CIME", "contact": "[email protected]", "is_internal": "True", "theme": "", "vulgarization": { "id": 1, "fr_label": "Tout public", "en_label": "General public" }, "registration": { "id": 3, "fr_label": "Entrée libre", "en_label": "Free" }, "keywords": "", "file": null, "icalendar_url": "https://memento.epfl.ch/event/export/118314/", "category": { "id": 1, "code": "CONF", "fr_label": "Conférences - Séminaires", "en_label": "Conferences - Seminars", "activated": true }, "academic_calendar_category": null, "domains": [], "mementos": [ "https://memento.epfl.ch/api/v1/mementos/1/?format=api", "https://memento.epfl.ch/api/v1/mementos/5/?format=api", "https://memento.epfl.ch/api/v1/mementos/8/?format=api", "https://memento.epfl.ch/api/v1/mementos/9/?format=api", "https://memento.epfl.ch/api/v1/mementos/110/?format=api", "https://memento.epfl.ch/api/v1/mementos/339/?format=api", "https://memento.epfl.ch/api/v1/mementos/284/?format=api", "https://memento.epfl.ch/api/v1/mementos/6/?format=api" ] } ] }
