Acute Hypoglycemia Induces Retinal Cell Death in Mouse
Event details
| Date | 09.12.2010 |
| Hour | 09:30 |
| Speaker | Dr. Raphael RODUIT, IROVISION, Sion, Switzerland |
| Location | |
| Category | Conferences - Seminars |
Glucose is the most important metabolic substrate of the retina and maintenance of normoglycemia is an essential challenge for diabetic patients. Glycemic excursions could lead to cardiovascular disease, nephropathy, neuropathy and retinopathy. A vast body of literature exists on hyperglycemia namely in the field of diabetic retinopathy, but very little is known about the deleterious effect of hypoglycemia. Therefore, we decided to study the role of acute hypoglycemia in mouse retina.
We showed that a 5-hour hyperinsulinemic/hypoglycemic clamp induces retinal cell death via caspase 3 activation and decrease of GSh content. In vitro experiments confirmed the low-glucose induction of 661W cell death via superoxide production and activation of caspase 3, which was also concomitant with a decrease of GSH content. Moreover, complementation with extracellular glutathione ethyl ester (GSHee) at low glucose restored GSH level and reduced apoptosis.
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