Biophysics at the truly single-molecule level
Event details
| Date | 02.02.2017 |
| Hour | 16:30 › 17:30 |
| Speaker | Dr. Jean-Nicolas Longchamp, University of Zurich |
| Location | |
| Category | Conferences - Seminars |
Abstract:
Imaging single proteins and protein complexes at atomic resolution has been a long-standing dream for advancing biophysics and structural biology and with this various fields in natural science. After a short introduction in the field, I will show how by means of preparative mass-spectrometry, for the specific selection and gentle deposition of individual proteins onto ultraclean freestanding graphene, and low-energy electron holography for the non-destructive imaging, the first images of single proteins at sub-nanometer resolution were recently obtained. I will then focus on the future perspectives for this novel technology, especially the fundamental biological questions that could be addressed as well as the remaining challenges to attain atomic resolution and 3D-imaging. Finally, I will show that low-energy electron holography has the potential for microseconds time-resolved experiments as for instance imaging proteins conformational changes in a pump-probe like experimental scheme.
Imaging single proteins and protein complexes at atomic resolution has been a long-standing dream for advancing biophysics and structural biology and with this various fields in natural science. After a short introduction in the field, I will show how by means of preparative mass-spectrometry, for the specific selection and gentle deposition of individual proteins onto ultraclean freestanding graphene, and low-energy electron holography for the non-destructive imaging, the first images of single proteins at sub-nanometer resolution were recently obtained. I will then focus on the future perspectives for this novel technology, especially the fundamental biological questions that could be addressed as well as the remaining challenges to attain atomic resolution and 3D-imaging. Finally, I will show that low-energy electron holography has the potential for microseconds time-resolved experiments as for instance imaging proteins conformational changes in a pump-probe like experimental scheme.
Practical information
- Expert
- Free
- This event is internal
Organizer
- Prof. Benoit Deveaud, Insitute of Physics
Contact
- Blandine Jérôme