DNA mapping using super resolution fluorescence microscopy
Event details
| Date | 02.11.2016 |
| Hour | 18:00 |
| Speaker | Dr Jochem Deen (LOB/LBEN) |
| Location |
cm 1105
|
| Category | Conferences - Seminars |
You are cordially invited to the monthly Pizza-Optics-Beer (POB) seminar, organized by the EPFL Photonics Chapter (EPC). Please confirm your participation in the doodle. Abstract: DNA mapping is a method to locate the physical location of a small sequence on a DNA molecule. In the old days this DNA map was used as a framework for DNA sequencing. Back then, the method consisted of treating the DNA with a restriction enzyme that cuts the DNA at specific small sequences and reading out the pattern with a gel or a fluorescence microscope. However, this approach was fundamentally limited in resolution and has been used less and less over the years. In my research I focus on a the attachment of fluorescent molecules to the DNA rather than cutting it. The position of the label can then be determined using super-resolution fluorescence microscopy techniques. This could allow for a far higher density of labels and resolution of localisation. The end result of this approach will be a sequence-specific pattern visible on the microscope. The ultimate goal is to use this pattern and match it to known sequences in order to determine the species from which the DNA molecule originates. This would be especially useful for the recognition of bacterial species, which can now only be approximately categorized with current techniques.
Practical information
- Informed public
- Free
- This event is internal
Organizer
- EPFL Photonics Chapter