EPFL BioE Talks SERIES "Exploring and Exploiting the Secretory Pathway in Mammalian Cells"
Event details
| Date | 04.11.2024 |
| Hour | 12:15 › 13:15 |
| Speaker | Prof. Franck Perez, Institut Curie, Paris (FR) |
| Location | Online |
| Category | Conferences - Seminars |
| Event Language | English |
WEEKLY EPFL BIOE TALKS SERIES (sandwiches provided)
Abstract:
To regulate their homeostasis – to exchange signals with the extracellular environment or migrate for example – cells need to address particular proteins and lipids toward specific compartments in a tightly controlled way. Golgi-dependent trafficking plays an essential role in these processes but although our knowledge about the mechanisms of intra-Golgi transport and sorting has dramatically increased over the past two decades, the underlying complexity of transport routes, in term of mechanism and dynamics, is only starting to emerge.
Over the years, we developed several versatile assays to monitor and quantify transport of cargos to the cell surface like the RUSH assay and the CATCHFIRE system that brings powerful ways to study and perturb cell dynamics in real-time. Recently, we applied these set of tools to understand better how the cell surface PD-L1 is translocated in the secretory pathway and transported to the cell surface. We also exploited our trafficking assays to develop novel translational approaches, screen for inhibitory molecules and and set-up tunable cell-based therapies.
Bio:
Franck Perez is Research Director at CNRS and Chairman of the "Cell Biology and Cancer" Unit of the Institut Curie in Paris. His group is studying intracellular trafficking with an important focus on the development of new methods and tools for Cell Biology studies. For example, he developed at the Institut Curie the use of automated cellular screening and co-founded a High Content screening platform (BioPhenics, directed by Elaine del Nery). In addition, his group created a novel trafficking assay and have strongly invested on the development of recombinant antibodies for cell biology. Notably, with Sandrine Moutel he developed a novel antibody library based on a humanized nanobody scaffold and created a therapeutic antibody platform to exploit and share these technologies.
Prior to being recruited by CNRS, F. Perez was Maître-Assistant at the University of Geneva in Switzerland, in the laboratory of Prof. T. E Kreis (1996-1999) where he worked on microtubule dynamics and intracellular trafficking. F. Perez received his PhD. in 1996 for his work in the laboratory of A. Prochiantz at the Ecole Normale Supérieure (Paris, France) on the regulation of neuroendocrine secretion and on the development of cell internalization peptides. Before his PhD., F. Perez graduated in Cell biology and Genetics at the University Paris VII.
F. Perez is an EMBO member, author of hundred and fifty articles and over twelve patents. He is the co-founder of four biotech companies (Emglev therapeutics (now Valour Bio), Honing Biosciences, The Twinkle Factory and Mnemo therapeutics).
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IN CASE you cannot attend in-person in the room, please make sure to
Abstract:
To regulate their homeostasis – to exchange signals with the extracellular environment or migrate for example – cells need to address particular proteins and lipids toward specific compartments in a tightly controlled way. Golgi-dependent trafficking plays an essential role in these processes but although our knowledge about the mechanisms of intra-Golgi transport and sorting has dramatically increased over the past two decades, the underlying complexity of transport routes, in term of mechanism and dynamics, is only starting to emerge.
Over the years, we developed several versatile assays to monitor and quantify transport of cargos to the cell surface like the RUSH assay and the CATCHFIRE system that brings powerful ways to study and perturb cell dynamics in real-time. Recently, we applied these set of tools to understand better how the cell surface PD-L1 is translocated in the secretory pathway and transported to the cell surface. We also exploited our trafficking assays to develop novel translational approaches, screen for inhibitory molecules and and set-up tunable cell-based therapies.
Bio:
Franck Perez is Research Director at CNRS and Chairman of the "Cell Biology and Cancer" Unit of the Institut Curie in Paris. His group is studying intracellular trafficking with an important focus on the development of new methods and tools for Cell Biology studies. For example, he developed at the Institut Curie the use of automated cellular screening and co-founded a High Content screening platform (BioPhenics, directed by Elaine del Nery). In addition, his group created a novel trafficking assay and have strongly invested on the development of recombinant antibodies for cell biology. Notably, with Sandrine Moutel he developed a novel antibody library based on a humanized nanobody scaffold and created a therapeutic antibody platform to exploit and share these technologies.
Prior to being recruited by CNRS, F. Perez was Maître-Assistant at the University of Geneva in Switzerland, in the laboratory of Prof. T. E Kreis (1996-1999) where he worked on microtubule dynamics and intracellular trafficking. F. Perez received his PhD. in 1996 for his work in the laboratory of A. Prochiantz at the Ecole Normale Supérieure (Paris, France) on the regulation of neuroendocrine secretion and on the development of cell internalization peptides. Before his PhD., F. Perez graduated in Cell biology and Genetics at the University Paris VII.
F. Perez is an EMBO member, author of hundred and fifty articles and over twelve patents. He is the co-founder of four biotech companies (Emglev therapeutics (now Valour Bio), Honing Biosciences, The Twinkle Factory and Mnemo therapeutics).
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IN CASE you cannot attend in-person in the room, please make sure to
- send D. Reinhard a note well ahead of time (ideally before seminar day), informing that you plan to attend the talk online, and, during seminar:
- be signed in on Zoom with a recognizable user name (not any alias making it difficult or impossible to identify you).
Practical information
- Informed public
- Registration required
Organizer
- Profs. Gisou van der Goot and Giovanni D'Angelo, EPFL
Contact
- Institute of Bioengineering (IBI), Dietrich REINHARD